ArticlePloS one2012
Early cell death detection with digital holographic microscopy.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 192 citations in OpenAlex.
- Digital Holographic Microscopy for Phenotypic Profiling of Adherent Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Determination of Drug Sensitivity in Patient Derived Models of Breast Cancer by Multiparametric QPI.bioRxiv : the preprint server for biology · 2025Article
- Measurement of HbA1c with a new phase function using RBC phase image from a low-cost digital holographic microscopy.Biotechnology letters · 2025Article
- Holographic Proteomics: A Review of Digital Holographic Microscopy Applications in Spatial Proteomics.Protein and peptide letters · 2025Review
- The Yin and Yang of hsa-miR-1244 expression levels during activation of the UPR control cell fate.Cell communication and signaling : CCS · 2024Article
- Long-term three-dimensional high-resolution imaging of live unlabeled small intestinal organoids via low-coherence holotomography.Experimental & molecular medicine · 2024Article
- Capturing cell morphology dynamics with high temporal resolution using single-shot quantitative phase gradient imaging.Journal of biomedical optics · 2024Article
- IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A.Cell communication and signaling : CCS · 2023Article
- Quantitative phase imaging for characterization of single cell growth dynamics.Lasers in medical science · 2023Review
- Review
- Interlaboratory evaluation of a digital holographic microscopy-based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers.Drug delivery and translational research · 2022Article
- Review
- Chemotherapy drug potency assessment method of ovarian cancer cells by digital holography microscopy.Biomedical optics express · 2022Article
- Observational
- Article
- Live-dead assay on unlabeled cells using phase imaging with computational specificity.Nature communications · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- Deep learning with digital holographic microscopy discriminates apoptosis and necroptosis.Cell death discovery · 2021Article
- Combining quantitative phase microscopy and laser-induced shockwave for the study of cell injury.Biomedical optics express · 2021Article
- Machine learning based approach to pH imaging and classification of single cancer cells.APL bioengineering · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDigital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM) dedicated to early and label-free detection of cell death. METHODS AND
findingsWe provide quantitative measurements of phase signal obtained on mouse cortical neurons, and caused by early neuronal cell volume regulation triggered by excitotoxic concentrations of L-glutamate. We show that the efficiency of this early regulation of cell volume detected by DHM, is correlated with the occurrence of subsequent neuronal death assessed with the widely accepted trypan blue method for detection of cell viability.
conclusionsThe determination of the phase signal by DHM provides a simple and rapid optical method for the early detection of cell death.
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