ArticleCold Spring Harbor protocols2012
Colocalization of fluorescent probes: accurate and precise registration with nanometer resolution.
Article in Cold Spring Harbor protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Simple and versatile imaging of genomic loci in live mammalian cells and early pre-implantation embryos using CAS-LiveFISH.Scientific reports · 2021Article
- Simultaneous tracking of two motor domains reveals near simultaneous steps and stutter steps of myosin 10 on actin filament bundles.Biochemical and biophysical research communications · 2020Article
- Multicolor single-particle reconstruction of protein complexes.Nature methods · 2018Article
- Mechanisms of inside-out signaling of the high-affinity IgG receptor FcγRI.Science signaling · 2018Article
- Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions.Nature communications · 2017Article
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- Transcriptional bursting is intrinsically caused by interplay between RNA polymerases on DNA.Nature communications · 2016Article
- Article
- Multi-wavelength single-molecule fluorescence analysis of transcription mechanisms.Methods (San Diego, Calif.) · 2015Article
- A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics.PLoS genetics · 2015Article
- Bending the rules: widefield microscopy and the Abbe limit of resolution.Journal of cellular physiology · 2014Review
- Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.Nano letters · 2013Article
- Article
- Single-molecule high-resolution colocalization of single probes.Cold Spring Harbor protocols · 2012Article
- Single mRNA tracking in live cells.Methods in enzymology · 2010Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Colocalization of fluorescent probes is commonly used in cell biology to discern the proximity of two proteins in the cell. Considering that the resolution limit of optical microscopy is on the order of 250 nm, there has not been a need for high-resolution colocalization techniques. However, with the advent of higher resolution techniques for cell biology and single-molecule biophysics, colocalization must also improve. For diffraction-limited applications, a geometric transformation (i.e., translation, scaling, and rotation) is typically applied to one color channel to align it with the other; however, to achieve high-resolution colocalization, this is not sufficient. Single-molecule high-resolution colocalization (SHREC) of single probes uses the local weighted mean transformation to achieve a colocalization resolution of at least 10 nm. This article describes the process of collecting a calibration data set of fiducials and the appropriate analysis to determine the transformation for colocalization.
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