ArticleJournal of clinical bioinformatics2012
Analysis of the salivary microbiome using culture-independent techniques.
Article in Journal of clinical bioinformatics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Microbiomic differences at cancer-prone oral mucosa sites with marijuana usage.Scientific reports · 2019Trial
- Trial
- Salivary microbiota profile in adult and children population according to active dentin caries: a metagenomic preliminary analysis.Frontiers in oral health · 2025Article
- Article
- Oral microbial taxa associated with risk for SARS-CoV-2 infection.Frontiers in oral health · 2022Article
- Old age and other factors associated with salivary microbiome variation.BMC oral health · 2021Article
- Comparison of Microbiome in Stimulated Saliva in Edentulous and Dentate Subjects.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Tobacco Smoking Affects the Salivary Gram-Positive Bacterial Population.Frontiers in public health · 2019Article
- Gender-Specific Associations Between Saliva Microbiota and Body Size.Frontiers in microbiology · 2019Article
- [The Microbiome - The Unscheduled Parameter for Future Therapies].Laryngo- rhino- otologie · 2018Review
- How low can we go? The implications of low bacterial load in respiratory microbiota studies.Pneumonia (Nathan Qld.) · 2018Review
- Microbial Signatures Associated with Oropharyngeal and Oral Squamous Cell Carcinomas.Scientific reports · 2017Article
- Differential responses of human dendritic cells to metabolites from the oral/airway microbiome.Clinical and experimental immunology · 2017Article
- Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples.Journal of oral microbiology · 2016Article
- Article
- Characterization of saliva microbiota's functional feature based on metagenomic sequencing.SpringerPlus · 2016Article
- Salivary bacterial fingerprints of established oral disease revealed by the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) technique.Journal of oral microbiology · 2016Article
- [The human virome].Revue francophone des laboratoires : RFL · 2015Article
- Article
- Emerging horizons of salivary diagnostics for periodontal disease.British dental journal · 2014Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe salivary microbiota is a potential diagnostic indicator of several diseases. Culture-independent techniques are required to study the salivary microbial community since many of its members have not been cultivated.
methodsWe explored the bacterial community composition in the saliva sample using metagenomic whole genome shotgun (WGS) sequencing, the extraction of 16S rRNA gene fragments from metagenomic sequences (16S-WGS) and high-throughput sequencing of PCR-amplified bacterial 16S rDNA gene (16S-HTS) regions V1 and V3.
resultsThe hierarchical clustering of data based on the relative abundance of bacterial genera revealed that distances between 16S-HTS datasets for V1 and V3 regions were greater than those obtained for the same V region with different numbers of PCR cycles. Datasets generated by 16S-HTS and 16S-WGS were even more distant. Finally, comparison of WGS and 16S-based datasets revealed the highest dissimilarity.The analysis of the 16S-HTS, WGS and 16S-WGS datasets revealed 206, 56 and 39 bacterial genera, respectively, 124 of which have not been previously identified in salivary microbiomes. A large fraction of DNA extracted from saliva corresponded to human DNA. Based on sequence similarity search against completely sequenced genomes, bacterial and viral sequences represented 0.73% and 0.0036% of the salivary metagenome, respectively. Several sequence reads were identified as parts of the human herpesvirus 7.
conclusionsAnalysis of the salivary metagenome may have implications in diagnostics e.g. in detection of microorganisms and viruses without designing specific tests for each pathogen.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.