Evidence map›Paper›PMID 22299932›Full record

ArticleThe Review of scientific instruments2012

Total internal reflection fluorescence microscopy imaging-guided confocal single-molecule fluorescence spectroscopy.

Desheng Zheng, Leonora Kaldaras, H Peter Lu

Open access · bronzeAbstract read
In one paragraph

Article in The Review of scientific instruments, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.5field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Single-Molecule Electrochemistry on a Porous Silica-Coated Electrode.Journal of the American Chemical Society · 2017
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Desheng ZhengDepartment of Chemistry, Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, USA.
Leonora Kaldaras
H Peter Lu
Bowling Green State University · US

Funding

Manipulating Single-Molecule Enzyme Conformations and ActivitiesR01GM084402 · NIGMS · BOWLING GREEN STATE UNIVERSITY · PI LU, H PETER · 2010 to 2013
$1.2M
Single-Molecule Patch-Clamp FRET Imaging Microscopy in Living CellsR01GM098089 · NIGMS · BOWLING GREEN STATE UNIVERSITY · PI LU, H PETER · 2012 to 2017
$1.2M
NIGMS NIH HHS R01 GM084402NIGMS NIH HHS R01 GM098089
6 · The paper itself

Abstract

We have developed an integrated spectroscopy system combining total internal reflection fluorescence microscopy imaging with confocal single-molecule fluorescence spectroscopy for two-dimensional interfaces. This spectroscopy approach is capable of both multiple molecules simultaneously sampling and in situ confocal fluorescence dynamics analyses of individual molecules of interest. We have demonstrated the calibration with fluorescent microspheres, and carried out single-molecule spectroscopy measurements. This integrated single-molecule spectroscopy is powerful in studies of single molecule dynamics at interfaces of biological and chemical systems.

Indexed as

BiocatalysisCalibrationFeasibility StudiesGlassHorseradish PeroxidaseMicroscopy, FluorescenceMicrospheresOxazinesRhodaminesSpectrometry, FluorescenceStochastic ProcessesSurface PropertiesSystems IntegrationAmplex RedHorseradish PeroxidaseOxazinesrhodamine 6GRhodamines

Identifiers

PMID22299932
PMCPMC3277604
OpenAlexW2078503726

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.