ArticleThe Journal of biological chemistry2012
Bivalent carbohydrate binding is required for biological activity of Clitocybe nebularis lectin (CNL), the N,N'-diacetyllactosediamine (GalNAcβ1-4GlcNAc, LacdiNAc)-specific lectin from basidiomycete C. nebularis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 61 citations in OpenAlex.
- Crystal structure of a recombinant Agaricus bisporus mushroom mannose-binding protein with a longer C-terminal region.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Exposure to specific fungal lectins during adhesion impairs biofilm formation of Listeria on polystyrene.Microbial biotechnology · 2024Article
- Modulation ofFrontiers in cellular and infection microbiology · 2024Article
- The Crystal Structure ofToxins · 2022Article
- A concise review of mushrooms antiviral and immunomodulatory properties that may combat against COVID-19.Food chemistry advances · 2022Review
- Cocaprins, β-Trefoil Fold Inhibitors of Cysteine and Aspartic Proteases fromInternational journal of molecular sciences · 2022Article
- Article
- Prediction of the Mannose-Binding Site in the Agaricus bisporus Mannose-Binding Protein.The protein journal · 2021Article
- Lectin-Mediated Binding of EngineeredMicroorganisms · 2021Article
- Immunomodulatory Effects of Edible and Medicinal Mushrooms and Their Bioactive Immunoregulatory Products.Journal of fungi (Basel, Switzerland) · 2020Review
- A GM1b/asialo-GM1 oligosaccharide-binding R-type lectin from purplish bifurcate mussels Mytilisepta virgata and its effect on MAP kinases.The FEBS journal · 2020Article
- CNL-Molecules (Basel, Switzerland) · 2019Review
- Control of Anthracnose and Gray Mold in Pepper Plants Using Culture Extract of White-Rot Fungus and Active Compound Schizostatin.Mycobiology · 2019Article
- Functional Characterization of OXYL, A SghC1qDC LacNAc-specific Lectin from The Crinoid Feather StarMarine drugs · 2019Article
- Cytotoxic protein from the mushroomProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Insights into the effects of glycosylation and the monosaccharide-binding activity of the plant lectin CrataBL.Glycoconjugate journal · 2017Article
- Fungal lectin MpL enables entry of protein drugs into cancer cells and their subcellular targeting.Oncotarget · 2017Article
- Agarolytic bacterium Persicobacter sp. CCB-QB2 exhibited a diauxic growth involving galactose utilization pathway.MicrobiologyOpen · 2017Article
- Molecular Basis for Recognition of the Cancer Glycobiomarker, LacdiNAc (GalNAc[β1→4]GlcNAc), by Wisteria floribunda Agglutinin.The Journal of biological chemistry · 2016Article
- Combining 3D structure with glycan array data provides insight into the origin of glycan specificity.Glycobiology · 2016Article
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9 authors at 5 institutions in 3 countries.
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Abstract
Lectins are carbohydrate-binding proteins that exert their biological activity by binding to specific cell glycoreceptors. We have expressed CNL, a ricin B-like lectin from the basidiomycete Clitocybe nebularis in Escherichia coli. The recombinant lectin, rCNL, agglutinates human blood group A erythrocytes and is specific for the unique glycan N,N'-diacetyllactosediamine (GalNAcβ1-4GlcNAc, LacdiNAc) as demonstrated by glycan microarray analysis. We here describe the crystal structures of rCNL in complex with lactose and LacdiNAc, defining its interactions with the sugars. CNL is a homodimeric lectin, each of whose monomers consist of a single ricin B lectin domain with its β-trefoil fold and one carbohydrate-binding site. To study the mode of CNL action, a nonsugar-binding mutant and nondimerizing monovalent CNL mutants that retain carbohydrate-binding activity were prepared. rCNL and the mutants were examined for their biological activities against Jurkat human leukemic T cells and the hypersensitive nematode Caenorhabditis elegans mutant strain pmk-1. rCNL was toxic against both, although the mutants were inactive. Thus, the bivalent carbohydrate-binding property of homodimeric CNL is essential for its activity, providing one of the rare pieces of evidence that certain activities of lectins are associated with their multivalency.
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