ArticleMolecular biology of the cell2012
GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- GPI transamidase complex is required for primordial germ cell migration and development in zebrafish.Journal of molecular cell biology · 2025Article
- Dissecting the Neuronal Contributions of the Lipid Regulator NHR-49 Function in Lifespan and Behavior inLife (Basel, Switzerland) · 2023Article
- A conditional counterselectableiScience · 2022Article
- The genetic basis and adult reproductive consequences of developmental thermal plasticity.The Journal of animal ecology · 2022Article
- Glycosylphosphatidylinositol Anchor Biosynthesis Pathway-Related Protein GPI7 Is Required for the Vegetative Growth and Pathogenicity ofInternational journal of molecular sciences · 2022Article
- Vps13-like proteins provide phosphatidylethanolamine for GPI anchor synthesis in the ER.The Journal of cell biology · 2022Article
- Article
- The Caenorhabditis elegans CUB-like-domain containing protein RBT-1 functions as a receptor for Bacillus thuringiensis Cry6Aa toxin.PLoS pathogens · 2020Article
- Nanoscale analysis reveals no domain formation of glycosylphosphatidylinositol-anchored protein SAG1 in the plasma membrane of living Toxoplasma gondii.Histochemistry and cell biology · 2019Article
- Article
- Article
- N-glycomic Complexity in Anatomical Simplicity:Frontiers in molecular biosciences · 2019Review
- Regulation of TG accumulation and lipid droplet morphology by the novel TLDP1 inJournal of lipid research · 2017Article
- Zeamide, a Glycosylinositol Phosphorylceramide with the Novel Core Arap(1β→6)Ins Motif from the Marine Sponge Svenzea zeai.Molecules (Basel, Switzerland) · 2017Article
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- Glycosyl phosphatidylinositol anchor biosynthesis is essential for maintaining epithelial integrity during Caenorhabditis elegans embryogenesis.PLoS genetics · 2015Article
- RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.Glycobiology · 2015Article
- Worming our way in and out of the Caenorhabditis elegans germline and developing embryo.Traffic (Copenhagen, Denmark) · 2013Review
- Orphan enzymes in ether lipid metabolism.Biochimie · 2013Review
- Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylphosphatidylinositol (GPI)-anchor attachment is one of the most common posttranslational protein modifications. Using the nematode Caenorhabditis elegans, we determined that GPI-anchored proteins are present in germline cells and distal tip cells, which are essential for the maintenance of the germline stem cell niche. We identified 24 C. elegans genes involved in GPI-anchor synthesis. Inhibition of various steps of GPI-anchor synthesis by RNA interference or gene knockout resulted in abnormal development of oocytes and early embryos, and both lethal and sterile phenotypes were observed. The piga-1 gene (orthologue of human PIGA) codes for the catalytic subunit of the phosphatidylinositol N-acetylglucosaminyltransferase complex, which catalyzes the first step of GPI-anchor synthesis. We isolated piga-1-knockout worms and found that GPI-anchor synthesis is indispensable for the maintenance of mitotic germline cell number. The knockout worms displayed 100% lethality, with decreased mitotic germline cells and abnormal eggshell formation. Using cell-specific rescue of the null allele, we showed that expression of piga-1 in somatic gonads and/or in germline is sufficient for normal embryonic development and the maintenance of the germline mitotic cells. These results clearly demonstrate that GPI-anchor synthesis is indispensable for germline formation and for normal development of oocytes and eggs.
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