ArticlePlant molecular biology2012
Serotonin accumulation in transgenic rice by over-expressing tryptophan decarboxylase results in a dark brown phenotype and stunted growth.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 72 citations in OpenAlex.
- The Fungal Pathogen Fusarium graminearum Can Hydrolyse Tryptamine-Derived Hydroxy-Cinnamic Acid Amide Defence Compounds, and Generates Highly Increased Auxin Levels in Wheat via Copper Amine-Oxidases.Molecular plant pathology · 2026Article
- Engineering hormonal crosstalk to enhance serotonin/melatonin levels in rice.Nature communications · 2025Article
- Manipulation of the Expression of Tryptophan Decarboxylase Boosts Grain Functional Quality and Stress Resilience Capacity of Wheat.Plant biotechnology journal · 2025Article
- Metabolomics Revealed the Differential Metabolites of Different Broomcorn Millet Varieties in Shanxi.Food science & nutrition · 2025Article
- Role of Serotonin in Cadmium Mitigation in Plants.Plants (Basel, Switzerland) · 2025Review
- Structural variation in theMolecular breeding : new strategies in plant improvement · 2025Article
- Genetic dissection of a major locus SC9.1 conferring seed color in broomcorn millet (Panicum miliaceum).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Integrative transcriptome and metabolome evaluation of melanin biosynthesis inForestry research · 2025Article
- Pangenome analysis reveals genomic variations associated with domestication traits in broomcorn millet.Nature genetics · 2023Article
- A Comprehensive Genome-Wide Investigation of the Cytochrome 71 (Plants (Basel, Switzerland) · 2023Article
- Melatonin-mediated temperature stress tolerance in plants.GM crops & food · 2022Review
- OsbZIP18, a Positive Regulator of Serotonin Biosynthesis, Negatively Controls the UV-B Tolerance in Rice.International journal of molecular sciences · 2022Article
- Deciphering Molecular Host-Pathogen Interactions DuringFrontiers in plant science · 2021Article
- Melatonin Regulatory Mechanisms and Phylogenetic Analyses of Melatonin Biosynthesis Related Genes Extracted from Peanut under Salinity Stress.Plants (Basel, Switzerland) · 2020Article
- Lysine, Lysine-Rich, Serine, and Serine-Rich Proteins: Link Between Metabolism, Development, and Abiotic Stress Tolerance and the Role of ncRNAs in Their Regulation.Frontiers in plant science · 2020Review
- Comparative physiological responses and transcriptome analysis reveal the roles of melatonin and serotonin in regulating growth and metabolism in Arabidopsis.BMC plant biology · 2018Article
- A Connection between Lysine and Serotonin Metabolism in Rice Endosperm.Plant physiology · 2018Article
- TheFrontiers in plant science · 2018Article
- Genome-Wide Association Studies of Free Amino Acid Levels by Six Multi-Locus Models in Bread Wheat.Frontiers in plant science · 2018Article
- Evolutionary Trails of Plant Group II Pyridoxal Phosphate-Dependent Decarboxylase Genes.Frontiers in plant science · 2016Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mutant M47286 with a stunted growth, low fertility and dark-brown phenotype was identified from a T-DNA-tagged rice mutant library. This mutant contained a copy of the T-DNA tag inserted at the location where the expression of two putative tryptophan decarboxylase genes, TDC-1 and TDC-3, were activated. Enzymatic assays of both recombinant proteins showed tryptophan decarboxylase activities that converted tryptophan to tryptamine, which could be converted to serotonin by a constitutively expressed tryptamine 5' hydroxylase (T5H) in rice plants. Over-expression of TDC-1 and TDC-3 in transgenic rice recapitulated the stunted growth, darkbrown phenotype and resulted in a low fertility similar to M47286. The degree of stunted growth and dark-brown color was proportional to the expression levels of TDC-1 and TDC-3. The levels of tryptamine and serotonin accumulation in these transgenic rice lines were also directly correlated with the expression levels of TDC-1 and TDC-3. A mass spectrometry assay demonstrated that the darkbrown leaves and hulls in the TDC-overexpressing transgenic rice were caused by the accumulation of serotonin dimer and that the stunted growth and low fertility were also caused by the accumulation of serotonin and serotonin dimer, but not tryptamine. These results represent the first evidence that over-expression of TDC results in stunted growth, low fertility and the accumulation of serotonin, which when converted to serotonin dimer, leads to a dark brown plant color.
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