ArticleJournal of visualized experiments : JoVE2012
Parallel-plate flow chamber and continuous flow circuit to evaluate endothelial progenitor cells under laminar flow shear stress.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Isolation of functional human endothelial cells from small volumes of umbilical cord blood.Annals of biomedical engineering · 2013Trial
- TRPV4 Dominates High Shear-Induced Initial Traction Response and Long-Term Relaxation Over Piezo1.bioRxiv : the preprint server for biology · 2025Article
- A Multichamber Pulsating-Flow Device With Optimized Spatial Shear Stress and Pressure for Endothelial Cell Testing.Journal of biomechanical engineering · 2025Article
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Transient low shear-stress preconditioning influences long-term endothelial traction and alignment under high shear flow.American journal of physiology. Heart and circulatory physiology · 2024Article
- Adiposomes from Obese-Diabetic Individuals Promote Endothelial Dysfunction and Loss of Surface Caveolae.Cells · 2023Article
- Microplastic-Free Microcapsules to Encapsulate Health-Promoting Limonene Oil.Molecules (Basel, Switzerland) · 2022Article
- In Vitro Flow Chamber Design for the Study of Endothelial Cell (Patho)Physiology.Journal of biomechanical engineering · 2022Review
- Shear type and magnitude affect aortic valve endothelial cell morphology, orientation, and differentiation.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress.STAR protocols · 2021Article
- Investigation of Wall Shear Stress in Cardiovascular Research and in Clinical Practice-From Bench to Bedside.International journal of molecular sciences · 2021Review
- Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel.Materials (Basel, Switzerland) · 2021Article
- Effect of Flow-Induced Shear Stress in Nanomaterial Uptake by Cells: Focus on Targeted Anti-Cancer Therapy.Cancers · 2020Review
- MechanoBioTester: A Decoupled Multistimulus Cell Culture Device for Studying Complex Microenvironments In Vitro.ACS biomaterials science & engineering · 2020Article
- Staphylococcus aureus adhesion in endovascular infections is controlled by the ArlRS-MgrA signaling cascade.PLoS pathogens · 2019Article
- Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow.Bio-protocol · 2019Article
- A portable device for studying the effects of fluid flow on degradation properties of biomaterials inside cell incubators.Regenerative biomaterials · 2019Article
- Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers.Journal of visualized experiments : JoVE · 2018Article
- Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System.Journal of visualized experiments : JoVE · 2018Article
- Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
The overall goal of this method is to describe a technique to subject adherent cells to laminar flow conditions and evaluate their response to well quantifiable fluid shear stresses. Our flow chamber design and flow circuit (Fig. 1) contains a transparent viewing region that enables testing of cell adhesion and imaging of cell morphology immediately before flow (Fig. 11A, B), at various time points during flow (Fig. 11C), and after flow (Fig. 11D). These experiments are illustrated with human umbilical cord blood-derived endothelial progenitor cells (EPCs) and porcine EPCs. This method is also applicable to other adherent cell types, e.g. smooth muscle cells (SMCs) or fibroblasts. The chamber and all parts of the circuit are easily sterilized with steam autoclaving. In contrast to other chambers, e.g. microfluidic chambers, large numbers of cells (> 1 million depending on cell size) can be recovered after the flow experiment under sterile conditions for cell culture or other experiments, e.g. DNA or RNA extraction, or immunohistochemistry (Fig. 11E), or scanning electron microscopy. The shear stress can be adjusted by varying the flow rate of the perfusate, the fluid viscosity, or the channel height and width. The latter can reduce fluid volume or cell needs while ensuring that one-dimensional flow is maintained. It is not necessary to measure chamber height between experiments, since the chamber height does not depend on the use of gaskets, which greatly increases the ease of multiple experiments. Furthermore, the circuit design easily enables the collection of perfusate samples for analysis and/or quantification of metabolites secreted by cells under fluid shear stress exposure, e.g. nitric oxide (Fig. 12).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.