ReviewBiotechnology advances
Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks.
Review in Biotechnology advances. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 88 citations in OpenAlex.
- Multimodal High-Throughput Screening Raman Spectroscopy for Label-Free Single-Cell Characterization of Recombinant Protein Production in Baculovirus Expression Vector Systems.Analytical chemistry · 2026Article
- CRISPR/Cas9-Driven Engineering of AcMNPV Using Dual gRNA for Optimized Recombinant Protein Production.Viruses · 2025Article
- Effect of glycosylation on protein folding: From biological roles to chemical protein synthesis.iScience · 2025Review
- Equi-MOI ratio for rapid baculovirus-mediated multiprotein co-expression in insect cells integrating selenomethionine for structural studies.FEBS open bio · 2025Article
- An automated and high-throughput approach for enhanced precision of adenoviral titering.Molecular therapy. Methods & clinical development · 2025Article
- Multiplicity of infection and culture medium on the SARS-CoV-2 virus like-particles production by baculovirus/insect system.Biotechnology letters · 2025Article
- Co-expression of the RPS6KB1 and PDPK1 genes for production of activated p70S6K1 using bac-to-bac baculovirus expression system.Molecular biology reports · 2025Article
- Article
- Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression.Scientific reports · 2024Article
- Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems.Molecular biotechnology · 2024Article
- Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Generation of Complex Protein Structures by Coinfection with High-Quality Recombinant Baculovirus.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Sf9 Cells Metabolism and Viability When Coinfected with Two Monocistronic Baculoviruses to Produce Rabies Virus-like Particles.Molecular biotechnology · 2023Article
- Microscopic Observation of Membrane Fusion between Giant Liposomes and Baculovirus Budded Viruses Activated by the Release of a Caged Proton.Membranes · 2023Article
- Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression.ACS synthetic biology · 2023Article
- Article
- Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System.Viruses · 2022Article
- A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells.Protein expression and purification · 2021Review
- OsDER1 Is an ER-Associated Protein Degradation Factor That Responds to ER Stress.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The baculovirus expression vector system (BEVS) is a versatile and powerful platform for protein expression in insect cells. With the ability to approach similar post-translational modifications as in mammalian cells, the BEVS offers a number of advantages including high levels of expression as well as an inherent safety during manufacture and of the final product. Many BEVS products include proteins and protein complexes that require expression from more than one gene. This review examines the expression strategies that have been used to this end and focuses on the distinguishing features between those that make use of single polycistronic baculovirus (co-expression) and those that use multiple monocistronic baculoviruses (co-infection). Three major areas in which researchers have been able to take advantage of co-expression/co-infection are addressed, including compound structure-function studies, insect cell functionality augmentation, and VLP production. The core of the review discusses the parameters of interest for co-infection and co-expression with time of infection (TOI) and multiplicity of infection (MOI) highlighted for the former and the choice of promoter for the latter. In addition, an overview of modeling approaches is presented, with a suggested trajectory for future exploration. The review concludes with an examination of the gaps that still remain in co-expression/co-infection knowledge and practice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.