ArticleCell division2012
The yeast mitogen-activated protein kinase Slt2 is involved in the cellular response to genotoxic stress.
Article in Cell division, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 58 citations in OpenAlex.
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- A Multimodel Study of the Role of Novel PKC Isoforms in the DNA Integrity Checkpoint.International journal of molecular sciences · 2023Article
- Enhancement of HSA-pFSHβ production by disruptingFrontiers in microbiology · 2022Article
- The CWI Pathway: A Versatile Toolbox to Arrest Cell-Cycle Progression.Journal of fungi (Basel, Switzerland) · 2021Review
- Regulation of Pkc1 Hyper-Phosphorylation by Genotoxic Stress.Journal of fungi (Basel, Switzerland) · 2021Article
- Profiling the physiological pitfalls of anti-hepatitis C direct-acting agents in budding yeast.Microbial biotechnology · 2021Article
- Activation of a Mitogen-Activated Protein Kinase Hog1 by DNA Damaging Agent Methyl Methanesulfonate in Yeast.Frontiers in molecular biosciences · 2020Article
- Stressing out or stressing in: intracellular pathways for SAPK activation.Current genetics · 2019Review
- Intracellular mechanism by which genotoxic stress activates yeast SAPK Mpk1.Molecular biology of the cell · 2018Article
- The role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humans.FEBS open bio · 2018Article
- Vps74 Connects the Golgi Apparatus and Telomeres inG3 (Bethesda, Md.) · 2018Article
- MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD.Molecular cell · 2017Article
- A comparative study of the degradation of yeast cyclins Cln1 and Cln2.FEBS open bio · 2017Article
- Valproate inhibits MAP kinase signalling and cell cycle progression in S. cerevisiae.Scientific reports · 2016Article
- Erk5 contributes to maintaining the balance of cellular nucleotide levels and erythropoiesis.Cell cycle (Georgetown, Tex.) · 2015Article
- Article
- Protein kinase C controls activation of the DNA integrity checkpoint.Nucleic acids research · 2014Article
- Genotoxic stress prevents Ndd1-dependent transcriptional activation of G2/M-specific genes in Saccharomyces cerevisiae.Molecular and cellular biology · 2014Article
- DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast.DNA repair · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe maintenance of genomic integrity is essential for cell viability. Complex signalling pathways (DNA integrity checkpoints) mediate the response to genotoxic stresses. Identifying new functions involved in the cellular response to DNA-damage is crucial. The Saccharomyces cerevisiae SLT2 gene encodes a member of the mitogen-activated protein kinase (MAPK) cascade whose main function is the maintenance of the cell wall integrity. However, different observations suggest that SLT2 may also have a role related to DNA metabolism.
resultsThis work consisted in a comprehensive study to connect the Slt2 protein to genome integrity maintenance in response to genotoxic stresses. The slt2 mutant strain was hypersensitive to a variety of genotoxic treatments, including incubation with hydroxyurea (HU), methylmetanosulfonate (MMS), phleomycin or UV irradiation. Furthermore, Slt2 was activated by all these treatments, which suggests that Slt2 plays a central role in the cellular response to genotoxic stresses. Activation of Slt2 was not dependent on the DNA integrity checkpoint. For MMS and UV, Slt2 activation required progression through the cell cycle. In contrast, HU also activated Slt2 in nocodazol-arrested cells, which suggests that Slt2 may respond to dNTP pools alterations. However, neither the protein level of the distinct ribonucleotide reductase subunits nor the dNTP pools were affected in a slt2 mutant strain. An analysis of the checkpoint function revealed that Slt2 was not required for either cell cycle arrest or the activation of the Rad53 checkpoint kinase in response to DNA damage. However, slt2 mutant cells showed an elongated bud and partially impaired Swe1 degradation after replicative stress, indicating that Slt2 could contribute, in parallel with Rad53, to bud morphogenesis control after genotoxic stresses.
conclusionsSlt2 is activated by several genotoxic treatments and is required to properly cope with DNA damage. Slt2 function is important for bud morphogenesis and optimal Swe1 degradation under replicative stress. The MAPK Slt2 appears as a new player in the cellular response to genotoxic stresses.
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