ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2012
Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- Frequency and Clinical Outcomes of Respiratory Infections in Children with Cancer Presenting with Febrile Illness.South Asian journal of cancer · 2025Article
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- Reduced graphene oxide-based field effect transistors for the detection of E7 protein of human papillomavirus in saliva.Analytical and bioanalytical chemistry · 2021Article
- Impact of respiratory viral panel testing on length of stay in pediatric cancer patients admitted with fever and neutropenia.Pediatric blood & cancer · 2020Article
- FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens.Diagnostic microbiology and infectious disease · 2019Article
- RNA respiratory viral infections in solid organ transplant recipients: Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice.Clinical transplantation · 2019Article
- Clinical correlation of influenza and respiratory syncytial virus load measured by digital PCR.PloS one · 2019Article
- Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia.Archives of virology · 2018Article
- Automated Real-Time Collection of Pathogen-Specific Diagnostic Data: Syndromic Infectious Disease Epidemiology.JMIR public health and surveillance · 2018Article
- Respiratory Viral Infections in Patients With Cancer or Undergoing Hematopoietic Cell Transplant.Frontiers in microbiology · 2018Review
- Comparison of xTAG respiratory virus panel and Verigene Respiratory Virus Plus for detecting influenza virus and respiratory syncytial virus.Journal of clinical laboratory analysis · 2015Article
- MoSAIC: Mobile Surveillance for Acute Respiratory Infections and Influenza-Like Illness in the Community.American journal of epidemiology · 2014Article
- Rapid diagnosis of influenza: state of the art.Clinics in laboratory medicine · 2014Review
- FilmArray® respiratory panel performance in respiratory samples from neonatal care units.Diagnostic microbiology and infectious disease · 2014Article
- Prospective evaluation for respiratory pathogens in children with sickle cell disease and acute respiratory illness.Pediatric blood & cancer · 2014Article
- The FilmArray® respiratory panel: an automated, broadly multiplexed molecular test for the rapid and accurate detection of respiratory pathogens.Expert review of molecular diagnostics · 2013Review
- Detection of respiratory coinfections in pediatric patients using a small volume polymerase chain reaction array respiratory panel: more evidence for combined droplet and contact isolation.American journal of infection control · 2013Article
- Molecular detection of respiratory viruses.Clinics in laboratory medicine · 2013Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe detection of viral respiratory tract infections has evolved greatly with the development of PCR based commercial systems capable of simultaneously detecting a wide variety of pathogens.
objectivesEvaluate the relative performance of two commercial broad range systems for the detection of viral agents in clinical respiratory tract specimens from immunocompromised children. STUDY
designA total of 176 patient samples were included in the analysis, representing only the first sample collected for each patient, and excluding failed reactions. Samples were de-identified and assayed in parallel using two different, broadly multiplexed PCR systems: ResPlex™ II Panel v2.0 (ResPlex), Qiagen, Hilden, Germany and FilmArray(®) Respiratory Panel (FilmArray), Idaho Technology Inc., Salt Lake City, UT. Method comparison was based upon pair-wise concordance of results according to patient age, viral target and number of targets detected.
resultsThe two systems showed an overall concordance, by patient, of 83.8% (p=0.0001). FilmArray detected at least one target in 68.8% of samples, while ResPlex detected at least one target in 56.8%. ResPlex failed to detect 20.7% of FilmArray positives, and FilmArray failed to detect 4% of ResPlex positives. The relative performance of each system (including which system detected a higher number of positive samples) varied when stratified by target viral pathogen.
conclusionsBroadly multiplexed PCR is an effective means of detecting large numbers of clinically relevant respiratory viral pathogens.
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