ArticleMicrobial biotechnology2012
A bile-inducible membrane protein mediates bifidobacterial bile resistance.
Article in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Probiotic potential of 'lactobacilli' isolated from traditionally fermented Ethiopian honey wine (Tej).BMC microbiology · 2025Article
- Identification of conserved genomic signatures specific to3 Biotech · 2023Article
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Article
- A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.Methods in molecular biology (Clifton, N.J.) · 2021Review
- The genus bifidobacterium: From genomics to functionality of an important component of the mammalian gut microbiota running title: Bifidobacterial adaptation to and interaction with the host.Computational and structural biotechnology journal · 2021Review
- A Gene Homologous to rRNA Methylase Genes Confers Erythromycin and Clindamycin Resistance in Bifidobacterium breve.Applied and environmental microbiology · 2018Article
- Gene Replacement and Fluorescent Labeling to Study the Functional Role of Exopolysaccharides inFrontiers in microbiology · 2017Article
- Genomic analysis of three Bifidobacterium species isolated from the calf gastrointestinal tract.Scientific reports · 2016Article
- Integrated transcriptomic and proteomic analysis of the bile stress response in a centenarian-originated probiotic Bifidobacterium longum BBMN68.Molecular & cellular proteomics : MCP · 2014Article
- Diversity, ecology and intestinal function of bifidobacteria.Microbial cell factories · 2014Review
- Article
- Experimental determination and characterization of the gap promoter of Bifidobacterium bifidum S17.Bioengineered · 2014Article
- Bifidobacteria-host interactions--an update on colonisation factors.BioMed research international · 2014Review
- Bile resistance mechanisms in Lactobacillus and Bifidobacterium.Frontiers in microbiology · 2013Article
- Accessing the inaccessible: molecular tools for bifidobacteria.Applied and environmental microbiology · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Bbr_0838 from Bifidobacterium breve UCC2003 is predicted to encode a 683 residue membrane protein, containing both a permease domain that displays similarity to transporters belonging to the major facilitator superfamily, as well as a CBS (cystathionine beta synthase) domain. The high level of similarity to bile efflux pumps from other bifidobacteria suggests a significant and general role for Bbr_0838 in bile tolerance. Bbr_0838 transcription was shown to be monocistronic and strongly induced upon exposure to bile. Further analysis delineated the transcriptional start site and the minimal region required for promoter activity and bile regulation. Insertional inactivation of Bbr_0838 in B. breve UCC2003 resulted in a strain, UCC2003:838(800) , which exhibited reduced survival upon cholate exposure as compared with the parent strain, a phenotype that was reversed when a functional, plasmid-encoded Bbr_0838 gene was introduced into UCC2003:838(800) . Transcriptome analysis of UCC2003:838(800) grown in the presence or absence of bile demonstrated that transcription of Bbr_0832, which is predicted to encode a macrolide efflux transporter gene, was significantly increased in the presence of bile, representing a likely compensatory mechanism for bile removal in the absence of Bbr_0838. This study represents the first in-depth analysis of a bile-inducible locus in bifidobacteria, identifying a key gene relevant for bifidobacterial bile tolerance.
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