Evidence map›Paper›PMID 22294903›Full record

ArticleSensors (Basel, Switzerland)2010

Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors.

Thongchai Kaewphinit, Somchai Santiwatanakul, Chamras Promptmas, Kosum Chansiri

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 41 citations in OpenAlex.

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  3. Charge Transfer Mechanism in Guanine-Based Self-Assembled Monolayers on a Gold Surface.Langmuir : the ACS journal of surfaces and colloids · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Thongchai KaewphinitDepartment of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand. tkaewphinit@yahoo.com
Somchai Santiwatanakul
Chamras Promptmas
Kosum Chansiri
Srinakharinwirot University · THMahidol University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.

Indexed as

Biosensing TechniquesDNA, BacterialElectrochemical TechniquesElectrodesGoldMycobacterium tuberculosisOligonucleotide ProbesSulfhydryl CompoundsDNA, BacterialGoldOligonucleotide ProbesSulfhydryl CompoundsMycobacterium tuberculosisnon-amplified genomic DNApiezoelectric biosensor

Identifiers

PMID22294903
PMCPMC3264455
OpenAlexW2166885005

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.