ArticleEnvironmental science and pollution research international2011
Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure.
Article in Environmental science and pollution research international, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Gonadal Atresia, Estrogen-Responsive, and Apoptosis-Specific mRNA Expression in Marine Mussels from the East China Coast: A Preliminary Study.Bulletin of environmental contamination and toxicology · 2022Article
- Comparative analysis of novel and common reference genes in adult tissues of the mussel Mytilus galloprovincialis.BMC genomics · 2022Article
- Long-term exposure of marine mussels to paracetamol: is time a healer or a killer?Environmental science and pollution research international · 2021Article
- The explosive trinitrotoluene (TNT) induces gene expression of carbonyl reductase in the blue mussel (Mytilus spp.): a new promising biomarker for sea dumped war relicts?Archives of toxicology · 2020Article
- Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis.Environmental science and pollution research international · 2020Article
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- Transcriptome-based identification of the optimal reference genes as internal controls for quantitative RT-PCR in razor clam (Sinonovacula constricta).Genes & genomics · 2018Article
- Identification and validation of reference genes for qPCR in the terrestrial gastropod Cepaea nemoralis.PloS one · 2018Article
- High estradiol exposure disrupts the reproductive cycle of the clam Ruditapes decussatus in a sex-specific way.Environmental science and pollution research international · 2017Article
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- Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla).Scientific reports · 2015Article
- Evaluation and identification of reliable reference genes for toxicological study in Caenorhabditis elegans.Molecular biology reports · 2014Article
- Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues.PloS one · 2013Article
- Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis.PloS one · 2013Article
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- Annual variation in the levels of transcripts of sex-specific genes in the mantle of the common mussel, Mytilus edulis.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue.
methodsTo facilitate this, six candidate genes, including several commonly used in the literature, were investigated in mussels at different stages of gametogenesis and following experimental exposure to a model estrogen (17b-estradiol). GeNorm and NormFinder softwares were employed to assess the stability of the reference genes.
resultsOur results demonstrate that the most stable reference genes are not the same in mussels at different stages of gametogenesis and in experimentally E2-exposed mussels. Interestingly, HEL (helicase) and ACT (actin) mRNA expression levels were most affected by the stage of gametogenesis and yet, in molluscan studies, ACT is possibly the most frequently used reference gene.
conclusionsWe demonstrate that the experimental results are highly dependent on the reference gene chosen and that statistically significant contrasting differences between sample groups are present or absent depending on the reference gene employed.
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