ArticlePlanta2012
Expression of mung bean pectin acetyl esterase in potato tubers: effect on acetylation of cell wall polymers and tuber mechanical properties.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Close contact: role of the extracellular matrix in cell adhesion and separation during plant development and reproduction.Plant reproduction · 2026Review
- Genome-wide analysis of the pectin acetylesterase (PAE) gene family in tomato and SlPAE16 functional characterization associated with pedicel abscission.BMC plant biology · 2026Article
- Plant Cell Wall PolysaccharidePlants (Basel, Switzerland) · 2024Review
- Low oxygen environment effect on the tomato cell wall composition during the fruit ripening process.BMC plant biology · 2024Article
- Silencing of a Pectin Acetylesterase (PAE) Gene Highly Expressed in Tobacco Pistils Negatively Affects Pollen Tube Growth.Plants (Basel, Switzerland) · 2023Article
- Cell wall ester modifications and volatile emission signatures of plant response to abiotic stress.Plant, cell & environment · 2022Article
- The evolving views of the simplest pectic polysaccharides: homogalacturonan.Plant cell reports · 2022Review
- Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency.Plant molecular biology · 2022Article
- Identification of Candidate Genes Associated With Tolerance to Apple Replant Disease by Genome-Wide Transcriptome Analysis.Frontiers in microbiology · 2022Article
- Bioinformatics analysis of3 Biotech · 2021Article
- The genetics and physiology of seed dormancy, a crucial trait in common bean domestication.BMC plant biology · 2021Article
- Systematic Analysis and Functional Validation of Citrus Pectin Acetylesterases (CsPAEs) Reveals that CsPAE2 Negatively Regulates Citrus Bacterial Canker Development.International journal of molecular sciences · 2020Article
- Balanced Xylan Acetylation is the Key Regulator of Plant Growth and Development, and Cell Wall Structure and for Industrial Utilization.International journal of molecular sciences · 2020Review
- Plant cell wall integrity maintenance in model plants and crop species-relevant cell wall components and underlying guiding principles.Cellular and molecular life sciences : CMLS · 2020Review
- Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte,Frontiers in plant science · 2020Article
- New Insights Into Wall PolysaccharideFrontiers in plant science · 2018Review
- Plant pectin acetylesterase structure and function: new insights from bioinformatic analysis.BMC genomics · 2017Article
- Article
- Extreme Hypoxic Conditions Induce Selective Molecular Responses and Metabolic Reset in Detached Apple Fruit.Frontiers in plant science · 2016Article
- Tuber shape and eye depth variation in a diploid family of Andean potatoes.BMC genetics · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mung bean (Vigna radiata) pectin acetyl esterase (CAA67728) was heterologously expressed in tubers of potato (Solanum tuberosum) under the control of the granule-bound starch synthase promoter or the patatin promoter in order to probe the significance of O-acetylation on cell wall and tissue properties. The recombinant tubers showed no apparent macroscopic phenotype. The enzyme was recovered from transgenic tubers using a high ionic strength buffer and the extract was active against a range of pectic substrates. Partial in vivo de-acetylation of cell wall polysaccharides occurred in the transformants, as shown by a 39% decrease in the degree of acetylation (DA) of tuber cell wall material (CWM). Treatment of CWM using a combination of endo-polygalacturonase and pectin methyl esterase extracted more pectin polymers from the transformed tissue compared to wild type. The largest effect of the pectin acetyl esterase (68% decrease in DA) was seen in the residue from this extraction, suggesting that the enzyme is preferentially active on acetylated pectin that is tightly bound to the cell wall. The effects of acetylation on tuber mechanical properties were investigated by tests of failure under compression and by determination of viscoelastic relaxation spectra. These tests suggested that de-acetylation resulted in a stiffer tuber tissue and a stronger cell wall matrix, as a result of changes to a rapidly relaxing viscoelastic component. These results are discussed in relation to the role of pectin acetylation in primary cell walls and its implications for industrial uses of potato fibres.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.