ArticleMolecules (Basel, Switzerland)2012
Modulation of the differentiation of dental pulp stem cells by different concentrations of β-glycerophosphate.
Article in Molecules (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Determination of the optimal concentration and duration of C5aR antagonist application in an inflammatory model of human dental pulp cells.FEBS open bio · 2023Article
- Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation.BMC oral health · 2021Article
- Evaluation of a collagen-bioaggregate composite scaffold in the repair of sheep pulp tissue.European oral research · 2021Article
- Comparative Proteomic and Metabolomic Analysis of Human Osteoblasts, Differentiated from Dental Pulp Stem Cells, Hinted Crucial Signaling Pathways Promoting Osteogenesis.International journal of molecular sciences · 2021Article
- The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells.Chemical reviews · 2020Review
- Time-dependent C5a and C5aR expression in dental pulp cells following stimulation with LTA and LPS.International journal of molecular medicine · 2019Article
- Structural features, processing mechanism and gene splice variants of dentin sialophosphoprotein.The Japanese dental science review · 2018Review
- Aspirin promotes osteogenic differentiation of human dental pulp stem cells.International journal of molecular medicine · 2018Article
- Endothelial cells and endothelin‑1 promote the odontogenic differentiation of dental pulp stem cells.Molecular medicine reports · 2018Article
- Cell-derived micro-environment helps dental pulp stem cells promote dental pulp regeneration.Cell proliferation · 2017Article
- Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans.International journal of molecular medicine · 2017Article
- Insulin-like growth factor 1 receptor and p38 mitogen-activated protein kinase signals inversely regulate signal transducer and activator of transcription 3 activity to control human dental pulp stem cell quiescence, propagation, and differentiation.Stem cells and development · 2014Article
- A histological comparison of a new pulp capping material and mineral trioxide aggregate in rat molars.Iranian endodontic journal · 2014Article
- Proliferation and osteo/odontoblastic differentiation of stem cells from dental apical papilla in mineralization-inducing medium containing additional KH(2)PO(4).Cell proliferation · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of β-glycerophosphate (β-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time- and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the β-GP concentration, and there was also an influence on MEPE expression when different concentrations of β-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, all experimental groups successfully generated mineralized nodules by Alizarin Red S and the 5 mM β-GP group formed more mineralized nodules quantitated using the CPC extraction method. In conclusion, there is a significant modulation of the β-GP during the differentiation of the DPSCs. The degree of odontoblast differentiation is β-glycerophosphate concentration dependent. A low concentration of β-GP (5 mM) has been shown to be the optimal concentration for stimulating the maturation of the DPSCs. Moreover, MEPE accompanied with DSP clearly demonstrates the degree of the differentiation.
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