ArticlePloS one2012
Genotypic and PFGE/MLVA analyses of Vibrio cholerae O1: geographical spread and temporal changes during the 2007-2010 cholera outbreaks in Thailand.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Repeated Extraneous Introductions of Cholera, Thailand, 2007-2025.Emerging infectious diseases · 2026Article
- Vibrio cholerae O1 El Tor strains linked to global cholera show region-specific patterns by pulsed-field gel electrophoresis.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2022Article
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- Phylogenetic Analysis Revealed the Dissemination of Closely Related EpidemicOpen forum infectious diseases · 2020Article
- Epidemiological and molecular forensics of cholera recurrence in Haiti.Scientific reports · 2019Article
- Comparison and Evaluation of the Molecular Typing Methods for ToxigenicFrontiers in microbiology · 2018Article
- Comparison of virulence gene profiles and genomic fingerprints ofTropical medicine and health · 2018Article
- Molecular Epidemiology of Cholera Outbreaks during the Rainy Season in Mandalay, Myanmar.The American journal of tropical medicine and hygiene · 2017Article
- Minimal genetic change in Vibrio cholerae in Mozambique over time: Multilocus variable number tandem repeat analysis and whole genome sequencing.PLoS neglected tropical diseases · 2017Article
- Comparative genome analysis of VSP-II and SNPs reveals heterogenic variation in contemporary strains of Vibrio cholerae O1 isolated from cholera patients in Kolkata, India.PLoS neglected tropical diseases · 2017Article
- Characterization of Vibrio cholerae Strains Isolated from the Nigerian Cholera Outbreak in 2010.Journal of clinical microbiology · 2016Article
- Molecular Epidemiology and Antibiotic Susceptibility of Vibrio cholerae Associated with a Large Cholera Outbreak in Ghana in 2014.PLoS neglected tropical diseases · 2016Article
- Characterization of 3 Megabase-Sized Circular Replicons from Vibrio cholerae.Emerging infectious diseases · 2015Article
- Cholera in Yangon, Myanmar, 2012-2013.Emerging infectious diseases · 2015Article
- Genetic traits of Vibrio cholerae O1 Haitian isolates that are absent in contemporary strains from Kolkata, India.PloS one · 2014Article
- Article
- Vibrio cholerae O1 isolate with novel genetic background, Thailand-Myanmar.Emerging infectious diseases · 2013Article
- Drug response and genetic properties of Vibrio cholerae associated with endemic cholera in north-eastern Thailand, 2003-2011.Journal of medical microbiology · 2013Article
- Outbreak-associated Vibrio cholerae genotypes with identical pulsotypes, Malaysia, 2009.Emerging infectious diseases · 2012Article
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Abstract
backgroundVibrio cholerae O1 El Tor dominated the seventh cholera pandemic which occurred in the 1960s. For two decades, variants of V. cholerae O1 El Tor that produce classical cholera toxin have emerged and spread globally, replacing the prototypic El Tor biotype. This study aims to characterize V. cholerae O1 isolates from outbreaks in Thailand with special reference to genotypic variations over time. METHODS/
findingsA total of 343 isolates of V. cholerae O1 from cholera outbreaks from 2007 to 2010 were investigated, and 99.4% were found to carry the classical cholera toxin B subunit (ctxB) and El Tor rstR genes. Pulsed-field gel electrophoresis (PFGE) differentiated the isolates into 10 distinct pulsotypes, clustered into two major groups, A and B, with an overall similarity of 88%. Ribotyping, multiple-locus variable-number tandem-repeat analysis (MLVA), and PCR to detect Vibrio seventh pandemic island II (VSP-II) related genes of randomly selected isolates from each pulsotype corresponded to the results obtained by PFGE. Epidemiological investigations revealed that MLVA type 2 was strongly associated with a cholera outbreak in northeastern Thailand in 2007, while MLVA type 7 dominated the outbreaks of the southern Gulf areas in 2009 and MLVA type 4 dominated the outbreaks of the central Gulf areas during 2009-2010. Only MLVA type 16 isolates were found in a Thai-Myanmar border area in 2010, whereas those of MLVA types 26, 39, and 41 predominated this border area in 2008. Type 39 then disappeared 1-2 years later as MLVA type 41 became prevalent. Type 41 was also found to infect an outbreak area.
conclusionsMLVA provided a high-throughput genetic typing tool for understanding the in-depth epidemiology of cholera outbreaks. Our epidemiological surveys suggest that some clones of V. cholerae O1 with similar but distinctive genetic traits circulate in outbreak sites, while others disappear over time.
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