ArticlePloS one2012
Reproducible and sustained regulation of Gαs signalling using a metazoan opsin as an optogenetic tool.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 87 citations in OpenAlex.
- Spectral tuning and signaling of diverse and far red-sensitive rhodopsins in mantis shrimp eyes.Science advances · 2026Article
- Discovery and design of photocyclic animal opsins: potential application to gene therapy from non-visual opsin research.Eye and vision (London, England) · 2025Review
- Optogenetic engineering for ion channel modulation.Current opinion in chemical biology · 2025Review
- Automated Plate Reader-Based Assays of Light-Activated GPCRs.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors.Nature communications · 2023Article
- Modulating signalling lifetime to optimise a prototypical animal opsin for optogenetic applications.Pflugers Archiv : European journal of physiology · 2023Article
- A visual opsin from jellyfish enables precise temporal control of G protein signalling.Nature communications · 2023Article
- Optogenetic activation of mGluR1 signaling in the cerebellum induces synaptic plasticity.iScience · 2023Article
- Functional optimization of light-activatable Opto-GPCRs: Illuminating the importance of the proximal C-terminus in G-protein specificity.Frontiers in cell and developmental biology · 2023Article
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
- Selective optogenetic control of GNature communications · 2022Article
- Optogenetics for light control of biological systems.Nature reviews. Methods primers · 2022Article
- Article
- Reverse optogenetics of G protein signaling by zebrafish non-visual opsin Opn7b for synchronization of neuronal networks.Nature communications · 2021Article
- Chimeric human opsins as optogenetic light sensitisers.The Journal of experimental biology · 2021Article
- Article
- Seasonal variation in UVA light drives hormonal and behavioural changes in a marine annelid via a ciliary opsin.Nature ecology & evolution · 2021Article
- Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein.eLife · 2020Article
- Recent advances in the use of genetically encodable optical tools to elicit and monitor signaling events.Current opinion in cell biology · 2020Review
- Light-Sensitive Membrane Proteins as Tools to Generate Precision Treatments.The Journal of membrane biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Originally developed to regulate neuronal excitability, optogenetics is increasingly also used to control other cellular processes with unprecedented spatiotemporal resolution. Optogenetic modulation of all major G-protein signalling pathways (Gq, Gi and Gs) has been achieved using variants of mammalian rod opsin. We show here that the light response driven by such rod opsin-based tools dissipates under repeated exposure, consistent with the known bleaching characteristics of this photopigment. We continue to show that replacing rod opsin with a bleach resistant opsin from Carybdea rastonii, the box jellyfish, (JellyOp) overcomes this limitation. Visible light induced high amplitude, reversible, and reproducible increases in cAMP in mammalian cells expressing JellyOp. While single flashes produced a brief cAMP spike, repeated stimulation could sustain elevated levels for 10s of minutes. JellyOp was more photosensitive than currently available optogenetic tools, responding to white light at irradiances ≥1 µW/cm(2). We conclude that JellyOp is a promising new tool for mimicking the activity of Gs-coupled G protein coupled receptors with fine spatiotemporal resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.