ArticlePloS one2012
Persistence of cytosine methylation of DNA following fertilisation in the mouse.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Epigenetic Modifications at the Center of the Barker Hypothesis and Their Transgenerational Implications.International journal of environmental research and public health · 2021Review
- Cellular Autofluorescence in Mouse Embryonic Fibroblasts Interferes with Antigen Detection Using Flow Cytometry.Journal of fluorescence · 2021Article
- Review
- Histone 3 lysine 9 acetylation is a biomarker of the effects of culture on zygotes.Reproduction (Cambridge, England) · 2017Article
- Specific and Stable Suppression of HIV Provirus Expression In Vitro by Chimeric Zinc Finger DNA Methyltransferase 1.Molecular therapy. Nucleic acids · 2017Article
- Measurement of global DNA methylation levels by flow cytometry in mouse fibroblasts.In vitro cellular & developmental biology. Animal · 2017Article
- Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI.Epigenetics & chromatin · 2017Article
- Assessment of 'one-step' versus 'sequential' embryo culture conditions through embryonic genome methylation and hydroxymethylation changes.Human reproduction (Oxford, England) · 2016Article
- Mapping global changes in nuclear cytosine base modifications in the early mouse embryo.Reproduction (Cambridge, England) · 2016Article
- Glucocorticoids and fetal programming part 1: Outcomes.Nature reviews. Endocrinology · 2014Review
- Article
- The intergenerational effects of early adversity.Progress in molecular biology and translational science · 2014Review
- The strong selective sweep candidate gene ADRA2C does not explain domestication related changes in the stress response of chickens.PloS one · 2014Article
- The maternal to zygotic transition in mammals.Molecular aspects of medicine · 2013Review
- Lessons from zebrafish on reprogramming the epigenetic code after fertilisation.Asian journal of andrology · 2013Article
- What obesity research tells us about epigenetic mechanisms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- 5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote.PloS one · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Normal development of the mammalian embryo requires epigenetic reprogramming of the genome. The level of cytosine methylation of CpG-rich (5meC) regions of the genome is a major epigenetic regulator and active global demethylation of 5meC throughout the genome is reported to occur within the first cell-cycle following fertilization. An enzyme or mechanism capable of catalysing such rapid global demethylation has not been identified. The mouse is a widely used model for studying developmental epigenetics. We have reassessed the evidence for this phenomenon of genome-wide demethylation following fertilisation in the mouse. We found when using conventional methods of immunolocalization that 5meC showed a progressive acid-resistant antigenic masking during zygotic maturation which gave the appearance of demethylation. Changing the unmasking strategy by also performing tryptic digestion revealed a persistence of a methylated state. Analysis of methyl binding domain 1 protein (MBD1) binding confirmed that the genome remained methylated following fertilisation. The maintenance of this methylated state over the first several cell-cycles required the actions of DNA methyltransferase activity. The study shows that any 5meC remodelling that occurs during early development is not explained by a global active loss of 5meC staining during the cleavage stage of development and global loss of methylation following fertilization is not a major component of epigenetic reprogramming in the mouse zygote.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.