ArticlePloS one2012
Microfluidic chips for in vivo imaging of cellular responses to neural injury in Drosophila larvae.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Article
- Peripheral Nerve Crush inCold Spring Harbor protocols · 2025Article
- Study of Axonal Injury and Degeneration inCold Spring Harbor protocols · 2025Article
- Immobilizing Second-InstarCold Spring Harbor protocols · 2025Article
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- Live-Imaging of Axonal Cargoes in Drosophila Brain Explants Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Stac protein regulates release of neuropeptides.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Modeling Neurodegenerative Disorders inInternational journal of molecular sciences · 2020Review
- Microfluidic Device for Microinjection ofMicromachines · 2020Article
- Long-term in vivo imaging of Drosophila larvae.Nature protocols · 2020Article
- Dstac Regulates Excitation-Contraction Coupling inFrontiers in physiology · 2020Article
- An Immobilization Technique for Long-Term Time-Lapse Imaging of ExplantedFrontiers in cell and developmental biology · 2020Article
- Frontiers in Microfluidics, a Teaching Resource Review.Bioengineering (Basel, Switzerland) · 2019Review
- Characterization of Proprioceptive System Dynamics in Behaving Drosophila Larvae Using High-Speed Volumetric Microscopy.Current biology : CB · 2019Article
- Microfluidics on the fly: Inexpensive rapid fabrication of thermally laminated microfluidic devices for live imaging and multimodal perturbations of multicellular systems.Biomicrofluidics · 2019Article
- Tools to reverse-engineer multicellular systems: case studies using the fruit fly.Journal of biological engineering · 2019Review
- Recording Neural Activity in Unrestrained Animals with Three-Dimensional Tracking Two-Photon Microscopy.Cell reports · 2018Article
- Microfluidic platforms for the study of neuronal injury in vitro.Biotechnology and bioengineering · 2018Review
- Ethanol stimulates the in vivo axonal movement of neuropeptide dense-core vesicles in Drosophila motor neurons.Journal of neurochemistry · 2018Article
- Microfluidics for mechanobiology of model organisms.Methods in cell biology · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
With powerful genetics and a translucent cuticle, the Drosophila larva is an ideal model system for live imaging studies of neuronal cell biology and function. Here, we present an easy-to-use approach for high resolution live imaging in Drosophila using microfluidic chips. Two different designs allow for non-invasive and chemical-free immobilization of 3(rd) instar larvae over short (up to 1 hour) and long (up to 10 hours) time periods. We utilized these 'larva chips' to characterize several sub-cellular responses to axotomy which occur over a range of time scales in intact, unanaesthetized animals. These include waves of calcium which are induced within seconds of axotomy, and the intracellular transport of vesicles whose rate and flux within axons changes dramatically within 3 hours of axotomy. Axonal transport halts throughout the entire distal stump, but increases in the proximal stump. These responses precede the degeneration of the distal stump and regenerative sprouting of the proximal stump, which is initiated after a 7 hour period of dormancy and is associated with a dramatic increase in F-actin dynamics. In addition to allowing for the study of axonal regeneration in vivo, the larva chips can be utilized for a wide variety of in vivo imaging applications in Drosophila.
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Registered trials
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