ArticlePloS one2012
A powerful method for transcriptional profiling of specific cell types in eukaryotes: laser-assisted microdissection and RNA sequencing.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Laser-Assisted Microdissection for Cell- and Tissue Type-Specific Transcriptome and RNA-Seq Analysis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Differential gene expression analysis identifies a group of defensin-like peptides from Solanum chacoense ovules with in vitro pollen tube attraction activity.Plant molecular biology · 2025Article
- Laser capture microdissection.Nature reviews. Methods primers · 2025Article
- Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopses.Communications biology · 2024Article
- Big Data in Gastroenterology Research.International journal of molecular sciences · 2023Review
- Genomic Signatures of Sexual Selection on Pollen-Expressed Genes in Arabis alpina.Molecular biology and evolution · 2022Article
- Formins control dynamics of F-actin in the central cell ofPlant signaling & behavior · 2021Article
- ARP2/3-independent WAVE/SCAR pathway and class XI myosin control sperm nuclear migration in flowering plants.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Single-cell RNA-seq analysis reveals ploidy-dependent and cell-specific transcriptome changes in Arabidopsis female gametophytes.Genome biology · 2020Article
- Review
- Characterization of Arabidopsis CYP79C1 and CYP79C2 by Glucosinolate Pathway Engineering inFrontiers in plant science · 2020Article
- Retro-ElementFrontiers in genetics · 2020Article
- Differential activity of F-box genes and E3 ligases distinguishes sexual versus apomictic germline specification in Boechera.Journal of experimental botany · 2019Article
- Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development.Malaria journal · 2019Article
- Abscisic acid is a substrate of the ABC transporter encoded by the durable wheat disease resistance gene Lr34.The New phytologist · 2019Article
- Epigenetic Regulation of Plant Gametophyte Development.International journal of molecular sciences · 2019Review
- A brief note on genes that trigger components of apomixis.Journal of biosciences · 2019Review
- Consistent Reanalysis of Genome-wide Imprinting Studies in Plants Using Generalized Linear Models Increases Concordance across Datasets.Scientific reports · 2019Article
- TheFrontiers in plant science · 2019Review
- ArabidopsisPlant physiology · 2018Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The acquisition of distinct cell fates is central to the development of multicellular organisms and is largely mediated by gene expression patterns specific to individual cells and tissues. A spatially and temporally resolved analysis of gene expression facilitates the elucidation of transcriptional networks linked to cellular identity and function. We present an approach that allows cell type-specific transcriptional profiling of distinct target cells, which are rare and difficult to access, with unprecedented sensitivity and resolution. We combined laser-assisted microdissection (LAM), linear amplification starting from <1 ng of total RNA, and RNA-sequencing (RNA-Seq). As a model we used the central cell of the Arabidopsis thaliana female gametophyte, one of the female gametes harbored in the reproductive organs of the flower. We estimated the number of expressed genes to be more than twice the number reported previously in a study using LAM and ATH1 microarrays, and identified several classes of genes that were systematically underrepresented in the transcriptome measured with the ATH1 microarray. Among them are many genes that are likely to be important for developmental processes and specific cellular functions. In addition, we identified several intergenic regions, which are likely to be transcribed, and describe a considerable fraction of reads mapping to introns and regions flanking annotated loci, which may represent alternative transcript isoforms. Finally, we performed a de novo assembly of the transcriptome and show that the method is suitable for studying individual cell types of organisms lacking reference sequence information, demonstrating that this approach can be applied to most eukaryotic organisms.
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