ArticleArchives of medical science : AMS2011
Schwann-like cell differentiation from rat bone marrow stem cells.
Article in Archives of medical science : AMS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Nerve regeneration using the Bio 3D nerve conduit fabricated with spheroids.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2022Review
- The Notch signalling pathway and miRNA regulation play important roles in the differentiation of Schwann cells from adipose-derived stem cells.Laboratory investigation; a journal of technical methods and pathology · 2022Article
- The Clinical Trials of Mesenchymal Stromal Cells Therapy.Stem cells international · 2021Review
- Effects of Cyclic Tensile Strain on Oxidative Stress and the Function of Schwann Cells.BioMed research international · 2018Article
- Low-frequency pulsed electromagnetic field pretreated bone marrow-derived mesenchymal stem cells promote the regeneration of crush-injured rat mental nerve.Neural regeneration research · 2018Article
- Article
- Effect of bone marrow derived mesenchymal stem cells on lung pathology and inflammation in ovalbumin-induced asthma in mouse.Iranian journal of basic medical sciences · 2016Article
- The Potential of Menstrual Blood-Derived Stem Cells in Differentiation to Epidermal Lineage: A Preliminary Report.World journal of plastic surgery · 2016Article
- Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells.Archives of medical science : AMS · 2015Article
- Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro.Stem cells international · 2015Article
- Phenomenon of painless knee in recurrent patellar dislocation in children.Archives of medical science : AMS · 2014Article
- Schwann-like cells seeded in acellular nerve grafts improve nerve regeneration.BMC musculoskeletal disorders · 2014Article
- Tissue engineered scaffolds in regenerative medicine.World journal of plastic surgery · 2014Review
- Therapeutic anti-tumor effect of exogenous apoptin driven by human survivin gene promoter in a lentiviral construct.Archives of medical science : AMS · 2013Article
- Does pretreatment of bone marrow mesenchymal stem cells with 5-azacytidine or double intravenous infusion improve their therapeutic potential for dilated cardiomyopathy?Medical science monitor basic research · 2013Article
- Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor.Archives of medical science : AMS · 2012Article
- Bio 3D Conduits Derived from Bone Marrow Stromal Cells Promote Peripheral Nerve Regeneration.Cell transplantationArticle
- NERVE ENDINGS AND VASCULAR SUPPLY IN SEMITENDINOSUS TENDON OF CEREBRAL PALSY CHILDREN.Acta ortopedica brasileiraArticle
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe main purpose of this study was differentiation of bone marrow stem cells (BMSCs) into Schwann-like cells and to determine the intensity of apoptosis in BMSCs during the differentiation process. MATERIAL AND
methodsBone marrow stem cells were isolated from the femur of adult rats and the identity of the undifferentiated BMSCs was confirmed by the detection of specific cell surface markers. The BMSCs were differentiated by sequential administration of β-mercaptoethanol and all-trans-retinoic acid as pre-inducer factors and a mixture of forskolin, basic fibroblast growth factor, platelet-derived growth factor-AA and heregulin-b1 as inducer factors. The immunocytochemical properties of differentiated Schwann-like cells were examined at a specified time point. Reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the gene expression of the undifferentiated and differentiated BMSCs. Cell apoptosis and viability were assessed with annexin V and propidium iodide double staining and dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT) assay.
resultsImmunocytochemistry staining and RT-PCR analysis revealed that the induced BMSCs exhibited Schwann cell-specific markers such as S-100, P75 and glial fibrillary acidic protein (GFAP) at the 14(th) day of differentiation. MTT assay and flow cytometry revealed that of the total BMSCs in the differentiation medium, 40% to 50% of the cells died by apoptosis, but the remaining cell population remained strongly attached to the substrate and differentiated.
conclusionsThese findings indicated that BMSCs could differentiate into Schwann-like cells. As a side effect of differentiation an increased cell death rate was noted and our findings indicate that the principle mode of cell death is by apoptosis.
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