ArticleThe Journal of cell biology1990
Identification of two HSP70-related Xenopus oocyte proteins that are capable of recycling across the nuclear envelope.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 108 citations in OpenAlex.
- Identification of HSPA8 as an interacting partner of MAB21L2 and an important factor in eye development.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Heat shock protein Grp78/BiP/HspA5 binds directly to TDP-43 and mitigates toxicity associated with disease pathology.Scientific reports · 2022Article
- Mitochondrial HSP70 Chaperone System-The Influence of Post-Translational Modifications and Involvement in Human Diseases.International journal of molecular sciences · 2021Review
- Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival.Scientific reports · 2018Article
- Nuclear Tau and Its Potential Role in Alzheimer's Disease.Biomolecules · 2016Review
- Participation of ezrin in bacterial uptake by trophoblast giant cells.Reproductive biology and endocrinology : RB&E · 2009Article
- Cooperation of salivary protein histatin 3 with heat shock cognate protein 70 relative to the G1/S transition in human gingival fibroblasts.The Journal of biological chemistry · 2009Article
- Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara americana.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- The 70-kD heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors.The Journal of cell biology · 2005Article
- Interaction between constitutively expressed heat shock protein, Hsc 70, and cysteine string protein is important for cortical granule exocytosis in Xenopus oocytes.The Journal of biological chemistry · 2005Article
- Role of chaperones in nuclear translocation and transactivation of steroid receptors.Endocrine · 2001Review
- Antibodies against a novel nucleolar and cytoplasmic antigen (p105-p42) present in the sera of patients with a subset of rheumatoid arthritis (RA) with signs of scleroderma.Clinical and experimental immunology · 1998Article
- A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm.Genes & development · 1998Article
- Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex.The Journal of cell biology · 1997Article
- Nuclear import of U snRNPs requires importin beta.The EMBO journal · 1997Article
- Subnuclear trafficking of glucocorticoid receptors in vitro: chromatin recycling and nuclear export.The Journal of cell biology · 1997Article
- Promoter- and cell-specific responses to sex steroids.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 1997Review
- Heat shock disassembles the nucleolus and inhibits nuclear protein import and poly(A)+ RNA export.The EMBO journal · 1996Article
- In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation.The Journal of cell biology · 1996Article
- ATP-dependent release of glucocorticoid receptors from the nuclear matrix.Molecular and cellular biology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Two 70-kD polypeptides, B3 and B4, are present in equivalent concentrations in the nucleus and cytoplasm of Xenopus oocytes. The objectives of this study were to determine if they (a) are members of the 70-kD family of heat shock proteins, and (b) recycle between the nuclear and cytoplasmic compartments. Evidence based on high-affinity binding to ATP, cross-reactivity of B3/B4-specific antibodies with rat hsc70, and a comparison of cyanogen bromide cleavage peptide maps with hsc70, verified that B3 and B4 are members of the 70-kD family of heat-shock proteins. Nuclear uptake studies were performed by microinjecting 125I-labeled B3/B4, rat hsc70, and BSA into the cytoplasm of oocytes, and examining their subsequent intracellular distributions. By 6 h postinjection, the nuclear concentration of B3/B4 and hsc70 were approximately 24-fold greater than BSA controls. It was also found that B3/B4-coated gold particles as large as 120A in diameter were able to enter the nucleus by passing through the pores. Nuclear efflux was analyzed by microinjecting the iodinated proteins directly into the oocyte nuclei. 2 h after nuclear injection, at least 46% of the B3/B4 and 60% of the hsc70 were found in the cytoplasmic fractions, compared with less than 10% for the BSA controls. Cell fusion experiments, in which labeled, anucleate oocyte vegetal hemispheres were fused, under oil, with nucleate unlabeled animal hemispheres, demonstrated that cytoplasmic B3 and B4 could enter the nucleus after equilibration was reached, arguing against the existence of separate nuclear and cytoplasmic populations. Collectively, these results show that B3, B4, and rat hsc70 are transported across the nuclear envelope and recycle between the nucleus and cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.