ArticleJournal of immunology (Baltimore, Md. : 1950)2012
Skin langerin+ dendritic cells transport intradermally injected anti-DEC-205 antibodies but are not essential for subsequent cytotoxic CD8+ T cell responses.
Article in Journal of immunology (Baltimore, Md. : 1950), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Neutrophils in the Bone Marrow Express DEC205, Guiding Their Migration to Inflamed Tissues.Immunology · 2025Article
- FcRn regulates antigen presentation in dendritic cells downstream of DEC205-targeted vaccines.NPJ vaccines · 2024Article
- Pseudomonas aeruginosa LecB suppresses immune responses by inhibiting transendothelial migration.EMBO reports · 2023Article
- Antigen targeting to dendritic cells: Still a place in future immunotherapy?European journal of immunology · 2022Review
- A population of dermal LangerinFrontiers in immunology · 2022Article
- (Not) Home alone: Antigen presenting cell - T Cell communication in barrier tissues.Frontiers in immunology · 2022Review
- Targeting human langerin promotes HIV-1 specific humoral immune responses.PLoS pathogens · 2021Article
- Intradermal Delivery of Dendritic Cell-Targeting Chimeric mAbs Genetically Fused to Type 2 Dengue Virus Nonstructural Protein 1.Vaccines · 2020Article
- A Liposomal Platform for Delivery of a Protein Antigen to Langerin-Expressing Cells.Biochemistry · 2019Article
- A Rift Valley fever virus Gn ectodomain-based DNA vaccine induces a partial protection not improved by APC targeting.NPJ vaccines · 2018Article
- Atopic dermatitis induces the expansion of thymus-derived regulatory T cells exhibiting a Th2-like phenotype in mice.Journal of cellular and molecular medicine · 2016Article
- Review
- Dendritic cell targeted vaccines: Recent progresses and challenges.Human vaccines & immunotherapeutics · 2016Article
- Targeting dendritic cells: a promising strategy to improve vaccine effectiveness.Clinical & translational immunology · 2016Review
- Article
- Functional Specialization of Skin Dendritic Cell Subsets in Regulating T Cell Responses.Frontiers in immunology · 2015Review
- Human skin dendritic cells can be targeted in situ by intradermal injection of antibodies against lectin receptors.Experimental dermatology · 2014Article
- Murine Langerin+ dermal dendritic cells prime CD8+ T cells while Langerhans cells induce cross-tolerance.EMBO molecular medicine · 2014Article
- Flt3L dependence helps define an uncharacterized subset of murine cutaneous dendritic cells.The Journal of investigative dermatology · 2014Article
- Immunogenicity of targeted lentivectors.Oncotarget · 2014Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 5 countries.
Funding
Abstract
Incorporation of Ags by dendritic cells (DCs) increases when Ags are targeted to endocytic receptors by mAbs. We have previously demonstrated in the mouse that mAbs against C-type lectins administered intradermally are taken up by epidermal Langerhans cells (LCs), dermal Langerin(neg) DCs, and dermal Langerin(+) DCs in situ. However, the relative contribution of these skin DC subsets to the induction of immune responses after Ag targeting has not been addressed in vivo. We show in this study that murine epidermal LCs and dermal DCs transport intradermally injected mAbs against the lectin receptor DEC-205/CD205 in vivo. Skin DCs targeted in situ with mAbs migrated through lymphatic vessels in steady state and inflammation. In the skin-draining lymph nodes, targeting mAbs were found in resident CD8α(+) DCs and in migrating skin DCs. More than 70% of targeted DCs expressed Langerin, including dermal Langerin(+) DCs and LCs. Numbers of targeted skin DCs in the nodes increased 2-3-fold when skin was topically inflamed by the TLR7 agonist imiquimod. Complete removal of the site where OVA-coupled anti-DEC-205 had been injected decreased endogenous cytotoxic responses against OVA peptide-loaded target cells by 40-50%. Surprisingly, selective ablation of all Langerin(+) skin DCs in Langerin-DTR knock-in mice did not affect such responses independently of the adjuvant chosen. Thus, in cutaneous immunization strategies where Ag is targeted to DCs, Langerin(+) skin DCs play a major role in transport of anti-DEC-205 mAb, although Langerin(neg) dermal DCs and CD8α(+) DCs are sufficient to subsequent CD8(+) T cell responses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.