ArticleJournal of the American Society for Mass Spectrometry2012
Characterizing peptide neutral losses induced by negative electron-transfer dissociation (NETD).
Article in Journal of the American Society for Mass Spectrometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Analytical applications of gas-phase ion chemistry enabled by mass spectrometry.Analytical methods : advancing methods and applications · 2026Review
- Implementation of Infrared-Activated Negative Electron Transfer Dissociation (IR-NETD) Using Xenon on a Quadrupole-Orbitrap-Quadrupole Linear Ion Trap Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2026Article
- Instrumentation at the Leading Edge of Proteomics.Analytical chemistry · 2024Review
- Phosphorothioate RNA Analysis by NETD Tandem Mass Spectrometry.Molecular & cellular proteomics : MCP · 2024Article
- Interactive Peptide Spectral Annotator: A Versatile Web-based Tool for Proteomic Applications.Molecular & cellular proteomics : MCP · 2019Article
- w-Type ions formed by electron transfer dissociation of Cys-containing peptides investigated by infrared ion spectroscopy.Journal of mass spectrometry : JMS · 2018Article
- Radical solutions: Principles and application of electron-based dissociation in mass spectrometry-based analysis of protein structure.Mass spectrometry reviews · 2018Review
- The Role of Electron Transfer Dissociation in Modern Proteomics.Analytical chemistry · 2018Review
- Sulfur Pentafluoride is a Preferred Reagent Cation for Negative Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2017Article
- pHisphorylation: the emergence of histidine phosphorylation as a reversible regulatory modification.Current opinion in cell biology · 2017Review
- Full-Featured Search Algorithm for Negative Electron-Transfer Dissociation.Journal of proteome research · 2016Article
- 213 nm Ultraviolet Photodissociation on Peptide Anions: Radical-Directed Fragmentation Patterns.Journal of the American Society for Mass Spectrometry · 2016Article
- Ion Activation Methods for Peptides and Proteins.Analytical chemistry · 2016Review
- The Negative Mode Proteome with Activated Ion Negative Electron Transfer Dissociation (AI-NETD).Molecular & cellular proteomics : MCP · 2015Article
- Direct identification of tyrosine sulfation by using ultraviolet photodissociation mass spectrometry.Journal of the American Society for Mass Spectrometry · 2014Article
- Article
- Chemical derivatization of peptide carboxyl groups for highly efficient electron transfer dissociation.Journal of the American Society for Mass Spectrometry · 2013Article
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Authors and funding
6 authors.
Funding
Abstract
We implemented negative electron-transfer dissociation (NETD) on a hybrid ion trap/Orbitrap mass spectrometer to conduct ion/ion reactions using peptide anions and radical reagent cations. In addition to sequence-informative ladders of a•- and x-type fragment ions, NETD generated intense neutral loss peaks corresponding to the entire or partial side-chain cleavage from amino acids constituting a given peptide. Thus, a critical step towards the characterization of this recently introduced fragmentation technique is a systematic study of synthetic peptides to identify common neutral losses and preferential fragmentation pathways. Examining 46 synthetic peptides with high mass accuracy and high resolution analysis permitted facile determination of the chemical composition of each neutral loss. We identified 19 unique neutral losses from 14 amino acids and three modified amino acids, and assessed the specificity and sensitivity of each neutral loss using a database of 1542 confidently identified peptides generated from NETD shotgun experiments employing high-pH separations and negative electrospray ionization. As residue-specific neutral losses indicate the presence of certain amino acids, we determined that many neutral losses have potential diagnostic utility. We envision this catalogue of neutral losses being incorporated into database search algorithms to improve peptide identification specificity and to further advance characterization of the acidic proteome.
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