ArticleParasitology research2012
Acanthamoeba spp. as vehicle and reservoir of adenoviruses.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.
- The Implication of Horizontal Gene Transfer BetweenPathogens (Basel, Switzerland) · 2026Pooled it
- Oxidative Stress and Redox Imbalance inInternational journal of molecular sciences · 2026Review
- Prevalence of HSV Genomic Signatures Among Acanthamoeba Hosts and Contaminated Lens Cases: A Molecular and Clinical Study.Investigative ophthalmology & visual science · 2025Article
- Utilizing Zebrafish Embryos for Replication of Tulane Virus: A Human Norovirus Surrogate.Food and environmental virology · 2024Article
- Article
- Viruses in Wastewater-A Concern for Public Health and the Environment.Microorganisms · 2024Review
- Waterborne Human Pathogenic Viruses in Complex Microbial Communities: Environmental Implication on Virus Infectivity, Persistence, and Disinfection.Environmental science & technology · 2022Review
- Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample.Scientific reports · 2021Article
- Microbial warfare in the wild-the impact of protists on the evolution and virulence of bacterial pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Extracellular amoebal-vesicles: potential transmission vehicles for respiratory viruses.NPJ biofilms and microbiomes · 2021Article
- A Systematic Review of Intracellular Microorganisms withinPathogens (Basel, Switzerland) · 2021Review
- Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology.Environmental research · 2020Article
- Occurrence, fates and potential treatment approaches for removal of viruses from wastewater: A review with emphasis on SARS-CoV-2.Journal of environmental chemical engineering · 2020Review
- Decay of infectious adenovirus and coliphages in freshwater habitats is differentially affected by ambient sunlight and the presence of indigenous protozoa communities.Virology journal · 2020Article
- Paradigms of Protist/Bacteria Symbioses Affecting Human Health:Frontiers in microbiology · 2020Review
- Acanthamoeba in Southeast Asia - Overview and Challenges.The Korean journal of parasitology · 2019Review
- Isolation and molecular identification of free-living amoebae from dishcloths in Tenerife, Canary Islands, Spain.Parasitology research · 2019Article
- Dual Role of Mechanisms Involved in Resistance to Predation by Protozoa and Virulence to Humans.Frontiers in microbiology · 2018Review
- Occurrence of Infected Free-Living Amoebae in Cooling Towers of Southern Brazil.Current microbiology · 2017Article
- Free-Living Amoebae as Hosts for and Vectors of Intracellular Microorganisms with Public Health Significance.Viruses · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenoviruses are important pathogens which are responsible for human enteritic, respiratory and eye infections. These viruses have been found to be prevalent in several natural and artificial water reservoirs worldwide. Free-living amoebae (FLA) have been recovered from similar water reservoirs, and it has been shown that FLA may act as reservoirs or vehicles of various microorganisms living in the same environment. To examine the ability of FLA to harbour adenoviruses, an in vitro study was conducted. Several Acanthamoeba strains were ‘co-cultivated’ with adenoviruses (adenoviruses 11 and 41), grown on A549 cells, using a proven test protocol. After phagocytosis and ingestion, the adenoviruses could be found within the cytoplasm of the Acanthamoeba trophozoites. The intake of the viruses into the cytoplasm of the trophozoites was demonstrated in an Acanthamoeba castellanii strain with the help of fluorescence microscopy and electron microscopy. An adenovirus DFA kit, which utilizes a direct immunofluorescent antibody technique for identifying adenovirus in infected tissue cultures, was used. In our study, it was demonstrated that adenoviruses were incorporated into the host amoebae (Acanthamoeba sp. Grp. II, three strains). So far, there were only a few publications concerning the relationship of free-living amoebae and viruses; only one of these described the detection of adenoviruses within acanthamoebae with molecular biological methods. We conducted this descriptive study to further examine the association between viable adenoviruses and FLA. To our knowledge, this is the first study to demonstrate directly the adenoviruses within FLA as vectors and vehicles. Therefore, we concluded that free-living amoebae appear able to act as carriers or vectors of the adenoviruses and thus may play a certain role in the dispersal of adenoviruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.