ArticleMarine biotechnology (New York, N.Y.)2012
Induction of autophagy by amino acid starvation in fish cells.
Article in Marine biotechnology (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Evaluating the Impact of Two Different Diets on the Protein Profile of the Brain, Liver, and Intestine of the Barramundi.Proteomes · 2026Article
- Article
- Characterization of threeFrontiers in physiology · 2024Article
- Transcriptome and DNA Methylation Responses in the Liver of Yellowfin Seabream Under Starvation Stress.Marine biotechnology (New York, N.Y.) · 2023Article
- AmAtg2B-Mediated Lipophagy Regulates Lipolysis of Pupae inInternational journal of molecular sciences · 2023Article
- An Integrated Bioinformatics Approach to Identify Network-Derived Hub Genes in Starving Zebrafish.Animals : an open access journal from MDPI · 2022Article
- Pharmacological and nutritional modulation of autophagy in a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1.In vitro cellular & developmental biology. Animal · 2020Article
- Effects of Starvation on Antioxidant-Related Signaling Molecules, Oxidative Stress, and Autophagy in Juvenile Chinese Perch Skeletal Muscle.Marine biotechnology (New York, N.Y.) · 2020Article
- Autophagy activation is required for homocysteine-induced apoptosis in bovine aorta endothelial cells.Heliyon · 2020Article
- The health and condition responses of Delta Smelt to fasting: A time series experiment.PloS one · 2020Article
- Genetically Based Physiological Responses to Overwinter Starvation in Gibel Carp (Frontiers in endocrinology · 2020Article
- A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress.Proteomes · 2019Article
- Inhibited Lipophagy Suppresses Lipid Metabolism in Zebrafish Liver Cells.Frontiers in physiology · 2019Article
- The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes.Frontiers in physiology · 2019Article
- Studying Autophagy in Zebrafish.Cells · 2017Review
- Phagocytic and Bactericidal Properties of Channel Catfish Peritoneal Macrophages Exposed toFrontiers in microbiology · 2017Article
- Lysine and Leucine Deficiencies Affect Myocytes Development and IGF Signaling in Gilthead Sea Bream (Sparus aurata).PloS one · 2016Article
- Autophagy as a defense strategy against stress: focus on Paracentrotus lividus sea urchin embryos exposed to cadmium.Cell stress & chaperones · 2016Review
- Macronutrient composition of the diet affects the feeding-mediated down regulation of autophagy in muscle of rainbow trout (O. mykiss).PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is well established as a starvation-induced process in yeast and mammalian cells and tissues. To elucidate the cellular mechanisms induced by starvation in fish, we characterized the induction of autophagy in cultured zebrafish cells under starvation conditions. As an autophagic marker protein, the microtubule-associated protein 1-light chain 3B protein (MAP1-LC3B) was cloned from the fish cells, and its expression and localization were characterized. In zebrafish embryonic (ZE) cells, posttranslational modifications produced two distinct forms of MAP1-LC3B, i.e., a cytosolic form and a membrane-bound form (types I and II, respectively). Immunofluorescence microscopy revealed fluorescently labeled autophagosomes in cells stably transfected with a green fluorescent protein (GFP)–MAP1-LC3B fusion protein and showed that this protein accumulated in punctate dots in a time-dependent manner in response to amino acid starvation. Starvation also induced the degradation of long-lived proteins. Treatment with 3-methyladenine and wortmannin, two class-III inhibitors of phosphoinositide 3-kinase (PI3K), repressed autophagy under starvation conditions, indicating that the PI3K class-III pathway regulates starvation-induced autophagy in fish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.