ArticlePhysics in medicine and biology2012
A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-squares deconvolution with Laguerre expansion.
Article in Physics in medicine and biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 117 citations in OpenAlex.
- Intraoperative Mapping of Parathyroid Glands Using Fluorescence Lifetime Imaging.The Journal of surgical research · 2021Trial
- Effects of surgical resection on tissue autofluorescence lifetime signatures in head and neck cancer: implications for intraoperative tumor margin assessment.Journal of biomedical optics · 2026Article
- Article
- Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.Journal of biomedical optics · 2026Article
- Fluorescence lifetime imaging of 5-ALA-induced PpIX and autofluorescence for detecting infiltrating glioblastoma margins in patients.Biomedical optics express · 2026Article
- Label-free fluorescence lifetime imaging for rapid discrimination of high-grade prostate cancer in fresh biopsy cores: a feasibility study.Journal of biomedical optics · 2026Article
- Fluorescence Properties of Collagen Types I-V: A Comprehensive Study of Spectral and Lifetime Characteristics.bioRxiv : the preprint server for biology · 2026Article
- Label-free fluorescence lifetime imaging can distinguish cancer from healthy tissue in spontaneously occurring canine oral tumors.Scientific reports · 2026Article
- Non-invasive Imaging Techniques: From Histology to In-vivo Imaging.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Assessing Intracellular Metabolism of Immune Cells In Situ in Live Zebrafish Larvae by Autofluorescence Lifetime Imaging Microscopy of NAD(P)H and FAD.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Phasor theory of fluorescence lifetime imaging utilized on a maximum range of frequencies for prostate tissue analysis.Biomedical optics express · 2025Article
- Multispectral laser-scanning pulse-sampling fluorescence lifetime system for large-scale tissue imaging.Optics letters · 2025Article
- Early Detection of Lymph Node Metastasis Using Primary Head and Neck Cancer Computed Tomography and Fluorescence Lifetime Imaging.Diagnostics (Basel, Switzerland) · 2024Article
- Dual modality intravascular catheter system combining pulse-sampling fluorescence lifetime imaging and polarization-sensitive optical coherence tomography.Biomedical optics express · 2024Article
- FLIm-Based in Vivo Classification of Residual Cancer in the Surgical Cavity During Transoral Robotic Surgery.Medical image computing and computer-assisted intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention · 2023Article
- Anatomy-Specific Classification Model Using Label-Free FLIm to Aid Intraoperative Surgical Guidance of Head and Neck Cancer.IEEE transactions on bio-medical engineering · 2023Article
- Pulse-sampling fluorescence lifetime imaging: evaluation of photon economy.Optics letters · 2023Article
- In vivo characterization of the human glioblastoma infiltrative edge with label-free intraoperative fluorescence lifetime imaging.Biomedical optics express · 2023Article
- Intraoperative detection of IDH-mutant glioma using fluorescence lifetime imaging.Journal of biophotonics · 2023Article
- Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model.Journal of biomedical optics · 2023Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We report a novel method for estimating fluorescence impulse response function (fIRF) from noise-corrupted time-domain fluorescence measurements of biological tissue. This method is based on the use of high-order Laguerre basis functions and a constrained least-squares approach that addresses the problem of overfitting due to increased model complexity. The new method was extensively evaluated on fluorescence data from simulation, fluorescent standard dyes, ex vivo tissue samples of atherosclerotic plaques and in vivo oral carcinoma. Current results demonstrate that this method allows for rapid and accurate deconvolution of multiple channel fluorescence decays without adaptively adjusting the Laguerre scale parameter. The appropriate choice of the scale parameter is essential for accurate estimation of the fIRF. The method described here is anticipated to play an important role in the development of computational techniques for real-time analysis of time-resolved fluorescence data from biological tissues and to support the advancement of fluorescence lifetime instrumentation for biomedical diagnostics by providing a means for on-line robust analysis of fluorescence decay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.