ArticleOsteoarthritis and cartilage2012
Response of cartilage and meniscus tissue explants to in vitro compressive overload.
Article in Osteoarthritis and cartilage, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- In Vitro Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants.Journal of visualized experiments : JoVE · 2025Article
- Healthy but not osteoarthritic human meniscus-derived matrix scaffolds promote meniscus repair.Frontiers in bioengineering and biotechnology · 2024Article
- Bio-orthogonal Click Chemistry Methods to Evaluate the Metabolism of Inflammatory Challenged Cartilage after Traumatic Overloading.ACS biomaterials science & engineering · 2022Article
- The MRI-based 3D morphologic changes of knee meniscus under knee weight-bearing and early flexion conditions.Scientific reports · 2021Observational
- Current Models for Development of Disease-Modifying Osteoarthritis Drugs.Tissue engineering. Part C, Methods · 2021Review
- Meniscus-Derived Matrix Bioscaffolds: Effects of Concentration and Cross-Linking on Meniscus Cellular Responses and Tissue Repair.International journal of molecular sciences · 2019Article
- Chondrocyte viability is lost during high-rate impact loading by transfer of amplified strain, but not stress, to pericellular and cellular regions.Osteoarthritis and cartilage · 2019Article
- Characterization of Injury Induced by Routine Surgical Manipulations of Nasal Septal Cartilage.JAMA facial plastic surgery · 2019Article
- Ex vivo physiological compression of human osteoarthritis cartilage modulates cellular and matrix components.PloS one · 2019Article
- Article
- Co-culture with infrapatellar fat pad differentially stimulates proteoglycan synthesis and accumulation in cartilage and meniscus tissues.Connective tissue research · 2017Article
- Inflammatory conditions partly impair the mechanically mediated activation of Smad2/3 signaling in articular cartilage.Arthritis research & therapy · 2016Article
- Inhibition of CDK9 prevents mechanical injury-induced inflammation, apoptosis and matrix degradation in cartilage explants.European cells & materials · 2016Article
- Meniscus is more susceptible than cartilage to catabolic and anti-anabolic effects of adipokines.Osteoarthritis and cartilage · 2015Article
- Mechanobiology of the meniscus.Journal of biomechanics · 2015Review
- Posttraumatic knee osteoarthritis following anterior cruciate ligament injury: Potential biochemical mediators of degenerative alteration and specific biochemical markers.Biomedical reports · 2015Article
- A high-throughput model of post-traumatic osteoarthritis using engineered cartilage tissue analogs.Osteoarthritis and cartilage · 2014Article
- Inhibition of cell-matrix adhesions prevents cartilage chondrocyte death following impact injury.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2014Article
- Analysis of the load on the knee joint and vertebral column with changes in squatting depth and weight load.Sports medicine (Auckland, N.Z.) · 2013Review
- Effect of partial and complete posterior cruciate ligament transection on medial meniscus: A biomechanical evaluation in a cadaveric model.Indian journal of orthopaedics · 2013Article
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2 authors.
Funding
Abstract
objectiveTo examine the relative susceptibility of cartilage and meniscus tissues to mechanical injury by applying a single, controlled overload and observing cellular, biochemical, and mechanical changes.
designCartilage and meniscus tissue explants in radial confinement were subjected to a range of injury by indenting to 40% strain at three different strain rates: 0.5%/s (slow), 5%/s (medium), or 50%/s (fast). Following injury, samples were cultured for either 1 or 9 days. Explants were assayed for cell metabolic activity, water content, and sulfated glycosaminoglycan (sGAG) content. Mechanical properties of explants were determined in torsional shear and unconfined compression. Conditioned medium was assayed for sGAG and lactate dehydrogenase (LDH) release.
resultsPeak injury force increased with strain rate but both tissues displayed little to no macroscopic damage. Cell metabolism was lowest in medium and fast groups on day 1. Cell lysis increased with peak injury force and loading rate in both tissues. In contrast, sGAG content and release did not significantly vary with loading rate. Additionally, mechanical properties did not significantly vary with loading rate in either tissue.
conclusionBy use of a custom confinement chamber, large peak forces were obtained without macroscopic destruction of the explants. At the loads achieved in this studied, cell damage was induced without detectable physical or compositional changes. These results indicate that sub-failure injury can induce biologic damage that may not be readily detected and could be an early event in osteoarthritis genesis.
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