ArticlePlasmid2012
Requirements for Borrelia burgdorferi plasmid maintenance.
Article in Plasmid, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Analysis of the BadR regulon in Borrelia burgdorferi.BMC microbiology · 2025Article
- Development and validation of systems for genetic manipulation of the Old World tick-borne relapsing fever spirochete, Borrelia duttonii.PLoS neglected tropical diseases · 2024Article
- Positioning of cellular components by the ParA/MinD family of ATPases.Current opinion in microbiology · 2024Review
- Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferi.PLoS genetics · 2023Article
- Multiple ParA/MinD ATPases coordinate the positioning of disparate cargos in a bacterial cell.Nature communications · 2023Article
- Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete.Nature communications · 2022Article
- Deletion of a Genetic Region of lp17 Affects Plasmid Copy Number inFrontiers in cellular and infection microbiology · 2022Article
- Article
- Primordial origin and diversification of plasmids in Lyme disease agent bacteria.BMC genomics · 2018Article
- Plasmid diversity and phylogenetic consistency in the Lyme disease agent Borrelia burgdorferi.BMC genomics · 2017Article
- Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy.Frontiers in microbiology · 2015Review
- Peaceful coexistence amongst Borrelia plasmids: getting by with a little help from their friends?Plasmid · 2013Review
- Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II.PLoS genetics · 2013Article
- Article
- Genetic Manipulation of Borrelia Spp.Current topics in microbiology and immunologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Borrelia burgdorferi has multiple linear and circular plasmids that are faithfully replicated and partitioned as the bacterium grows and divides. The low copy number of these replicons implies that active partitioning contributes to plasmid stability. Analyzing the requirements for plasmid replication and partition in B. burgdorferi is complicated by the complexity of the genome and the possibility that products may act in trans. Consequently, we have studied the replication-partition region (bbb10-13) of the B. burgdorferi 26kb circular plasmid (cp26) in Escherichia coli, by fusion with a partition-defective miniF plasmid. Our analysis demonstrated that bbb10, bbb11, and bbb13 are required for stable miniF maintenance, whereas bbb12 is dispensable. To validate these results, we attempted to inactivate two of these genes in B. burgdorferi. bbb12 mutants were obtained at a typical frequency, suggesting that the bbb12 product is dispensable for cp26 maintenance as well. We could not directly measure cp26 stability in the bbb12 mutant, because cp26 carries essential genes, and bacteria that have lost cp26 are inviable. Conversely, we were unable to inactivate bbb10 on cp26 of B. burgdorferi. Our results suggest that bbb12 is dispensable for cp26 maintenance, whereas bbb10, bbb11, and bbb13 play crucial roles in that process.
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Registered trials
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