ArticleGenomics, proteomics & bioinformatics2011
Evaluation of protocols used in 2-d electrophoresis for proteome analysis of young rice caryopsis.
Article in Genomics, proteomics & bioinformatics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 15 citations in OpenAlex.
- Two-Dimensional Gel Electrophoresis for Protein Separation of Plant Samples.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recent Molecular Aspects and Integrated Omics Strategies for Understanding the Abiotic Stress Tolerance of Rice.Plants (Basel, Switzerland) · 2023Review
- Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches.Proteome science · 2020Article
- Quantitative iTRAQ-based proteomic analysis of rice grains to assess high night temperature stress.Proteomics · 2017Article
- Comparative proteomic analysis of differentially expressed proteins in the early milky stage of rice grains during high temperature stress.Journal of experimental botany · 2014Article
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2 authors at 1 institution in 1 country.
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Abstract
In order to obtain a high-resolution electrophorogram of rice young panicle proteome, we evaluated various protocols commonly used in two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) of proteins, including gel staining protocol, pH range of immobilized pH gradient (IPG) strips and sample loading quantity. Results showed that a silver staining protocol using sensitized solution containing glacial acetic acid, sodium acetate and sodium thiosulfate (reported by Heukeshoven and Dernick in 1988) and a Coomassie Brilliant Blue staining method using solution containing G-250, ammonium sulfate and phosphoric acid (reported by Pink et al in 2010) demonstrated the superior staining effect. In addition, we also showed that higher resolution was achieved when IPG gel strip with pH range of 5-8 was used, compared to that with pH range of 4-7. Finally, the optimal loading quantity was determined as 130 μg using the 17 cm-long nonlinear IPG strip with pH 5-8 in combination with the silver nitrate staining protocol. The evaluated results would be helpful in proteome analysis of young rice caryopsis.
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