ArticleMethods in enzymology2012
Probes for intracellular RNA imaging in live cells.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Uncovering Molecular Quencher Effects on FRET Phenomena in Microsphere-Immobilized Probe Systems.Analytical chemistry · 2023Article
- Research progress of live-cell RNA imaging techniques.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells.Nucleic acids research · 2017Article
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.Chembiochem : a European journal of chemical biology · 2016Article
- Nucleic Acid-Based Nanodevices in Biological Imaging.Annual review of biochemistry · 2016Review
- RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells.Current protocols in chemical biology · 2016Article
- Can we observe changes in mRNA "state"? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes.The Analyst · 2016Review
- Dynamic visualization of transcription and RNA subcellular localization in zebrafish.Development (Cambridge, England) · 2015Article
- Post-transcriptional regulation of programmed cell death 4 (PDCD4) mRNA by the RNA-binding proteins human antigen R (HuR) and T-cell intracellular antigen 1 (TIA1).The Journal of biological chemistry · 2015Article
- Live-cell imaging of mammalian RNAs with Spinach2.Methods in enzymology · 2015Article
- MiR-21 in the extracellular vesicles (EVs) of cerebrospinal fluid (CSF): a platform for glioblastoma biomarker development.PloS one · 2013Article
- The dynamic pathway of nuclear RNA in eukaryotes.Nucleus (Austin, Tex.)Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
RNA localization, dynamics, and regulation are becoming increasingly important to our basic understanding of gene expression and RNA virus pathogenesis. An improved understanding of these processes will be necessary in order to identify new drug targets, as well as to create models of gene expression networks. Much of this new understanding will likely come from imaging studies of RNA, which can generate the spatiotemporal information necessary to characterize RNA within the cellular milieu. Ideally, this would be performed imaging native, nonengineered RNAs, but the approaches for performing these experiments are still evolving. In order for them to reach their potential, it is critical that they have characteristics that allow for the tracking of RNA throughout their life cycle. This chapter presents an overview of RNA imaging methodologies, and focuses on a single RNA sensitive method, employing exogenous probes, for imaging, native, nonengineered RNA in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.