ArticleJournal of proteome research2012
Vesicle and vesicle-free extracellular proteome of Paracoccidioides brasiliensis: comparative analysis with other pathogenic fungi.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
108 citing papers in PubMed, 181 citations in OpenAlex.
- Regulation of extracellular vesicles for protein secretion inMicrobial cell (Graz, Austria) · 2026Article
- Virulence and Stress-Related Proteins Are Differentially Enriched andJournal of fungi (Basel, Switzerland) · 2025Article
- Insights from Mass Spectrometry-Based Proteomics onJournal of fungi (Basel, Switzerland) · 2025Review
- The impact of Aureobasidium melanogenum cells and extracellular vesicles on human cell lines.Scientific reports · 2025Article
- Review
- Extracellular vesicles from virulentVirulence · 2024Article
- Fungal Extracellular Vesicle Proteins with Potential in Biological Interaction.Molecules (Basel, Switzerland) · 2024Review
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Thermotolerance in S. cerevisiae as a model to study extracellular vesicle biology.Journal of extracellular vesicles · 2024Article
- Importance of Non-Covalent Interactions in Yeast Cell Wall Molecular Organization.International journal of molecular sciences · 2024Review
- Proteomics ofJournal of fungi (Basel, Switzerland) · 2023Article
- Extracellular Vesicles and Bacteriophages: New Directions in Environmental Biocolloid Research.Environmental science & technology · 2023Review
- Immunomodulatory Potential of Fungal Extracellular Vesicles: Insights for Therapeutic Applications.Biomolecules · 2023Review
- Characteristics and potential clinical applications of the extracellular vesicles of human pathogenic Fungi.BMC microbiology · 2023Review
- Extracellular Vesicles of the Plant PathogenJournal of fungi (Basel, Switzerland) · 2023Article
- Fungal extracellular vesicle-mediated regulation: from virulence factor to clinical application.Frontiers in microbiology · 2023Review
- The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles.Frontiers in cellular and infection microbiology · 2023Article
- The emerging role of extracellular vesicles in fungi: a double-edged sword.Frontiers in microbiology · 2023Review
- Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.Fungal biology and biotechnology · 2022Article
- Challenges in Serologic Diagnostics of Neglected Human Systemic Mycoses: An Overview on Characterization of New Targets.Pathogens (Basel, Switzerland) · 2022Review
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Microorganisms release effector molecules that modulate the host machinery enabling survival, replication, and dissemination of a pathogen. Here we characterized the extracellular proteome of Paracoccidioides brasiliensis at its pathogenic yeast phase. Cell-free culture supernatants from the Pb18 isolate, cultivated in defined medium, were separated into vesicle and vesicle-free fractions, digested with trypsin, and analyzed by liquid chromatography-tandem mass spectrometry. In vesicle and vesicle-free preparations we identified, respectively, 205 and 260 proteins with two or more peptides, including 120 overlapping identifications. Almost 70% of the sequences were predicted as secretory, mostly using nonconventional secretory pathways, and many have previously been localized to fungal cell walls. A total of 72 proteins were considered as commonly transported by extracellular vesicles, considering that orthologues have been reported in at least two other fungal species. These sequences were mostly related to translation, carbohydrate and protein metabolism, oxidation/reduction, transport, response to stress, and signaling. This unique proteomic analysis of extracellular vesicles and vesicle-free released proteins in a pathogenic fungus provides full comparison with other fungal extracellular vesicle proteomes and broadens the current view on fungal secretomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.