ArticleNucleic acids research2012
DNA robustly stimulates FANCD2 monoubiquitylation in the complex with FANCI.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 87 citations in OpenAlex.
- FANCI: from genome guardian to translational therapeutic target.Frontiers in oncology · 2026Review
- Article
- Deciphering the role of post-translational modifications in fanconi anemia proteins and their influence on tumorigenesis.Cancer gene therapy · 2024Review
- The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export.Cell reports · 2024Article
- Structural and biochemical basis of interdependent FANCI-FANCD2 ubiquitination.The EMBO journal · 2023Article
- Fanconi anemia: current insights regarding epidemiology, cancer, and DNA repair.Human genetics · 2022Review
- The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination.Nature structural & molecular biology · 2022Article
- UBE2T regulates FANCI monoubiquitination to promote NSCLC progression by activating EMT.Oncology reports · 2022Article
- Regulation of the Fanconi Anemia DNA Repair Pathway by Phosphorylation and Monoubiquitination.Genes · 2021Review
- RNF168 E3 ligase participates in ubiquitin signaling and recruitment of SLX4 during DNA crosslink repair.Cell reports · 2021Article
- Structure of the FA core ubiquitin ligase closing the ID clamp on DNA.Nature structural & molecular biology · 2021Article
- WRNIP1 Is Recruited to DNA Interstrand Crosslinks and Promotes Repair.Cell reports · 2020Article
- Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA.EMBO reports · 2020Article
- Characterization of FANCL variants observed in patient cancer cells.Bioscience reports · 2020Article
- The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair.The Journal of biological chemistry · 2020Article
- Allosteric mechanism for site-specific ubiquitination of FANCD2.Nature chemical biology · 2020Article
- Rewiring E2 enzymes.Nature chemical biology · 2020Article
- FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair.Nature structural & molecular biology · 2020Article
- ATR-Mediated FANCI Phosphorylation Regulates Both Ubiquitination and Deubiquitination of FANCD2.Frontiers in cell and developmental biology · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
FANCI and FANCD2 form a complex, and play essential roles in the repair of interstrand DNA crosslinks (ICLs) by the Fanconi anemia (FA) pathway. FANCD2 is monoubiquitylated by the FA core complex, composed of 10 FA proteins including FANCL as the catalytic E3 subunit. FANCD2 monoubiquitylation can be reconstituted with purified minimal components, such as FANCI, E1, UBE2T (E2) and FANCL (E3) in vitro; however, its efficiency is quite low as compared to the in vivo monoubiquitylation of FANCD2. In this study, we found that various forms of DNA, such as single-stranded, double-stranded and branched DNA, robustly stimulated the FANCD2 monoubiquitylation in vitro up to a level comparable to its in vivo monoubiquitylation. This stimulation of the FANCD2 monoubiquitylation strictly required FANCI, suggesting that FANCD2 monoubiquitylation may occur in the FANCI-FANCD2 complex. A FANCI mutant that was defective in DNA binding was also significantly defective in FANCD2 monoubiquitylation in vitro. In the presence of 5' flapped DNA, a DNA substrate mimicking the arrested replication fork, about 70% of the input FANCD2 was monoubiquitylated, while less than 1% FANCD2 monoubiquitylation was observed in the absence of the DNA. Therefore, DNA may be the unidentified factor required for proper FANCD2 monoubiquitylation.
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