ArticleCardiovascular research2012
Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium current.
Article in Cardiovascular research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Cardiac Impulse Propagation: An Integrated View.JACC. Basic to translational science · 2026Review
- Estradiol and 3β-diol protect female cortical astrocytes by regulating connexin 43 Gap Junctions.Molecular and cellular endocrinology · 2023Article
- The intercalated disc: a unique organelle for electromechanical synchrony in cardiomyocytes.Physiological reviews · 2023Review
- Role of Connexin 43 phosphorylation on Serine-368 by PKC in cardiac function and disease.Frontiers in cardiovascular medicine · 2022Review
- Coupling between cardiac cells-An important determinant of electrical impulse propagation and arrhythmogenesis.Biophysics reviews · 2021Review
- Hypoplastic left heart syndrome (HLHS): molecular pathogenesis and emerging drug targets for cardiac repair and regeneration.Expert opinion on therapeutic targets · 2021Review
- Mechanisms of Arrhythmias in the Brugada Syndrome.International journal of molecular sciences · 2020Review
- Biomaterials and Culture Systems for Development of Organoid and Organ-on-a-Chip Models.Annals of biomedical engineering · 2020Review
- A study of the outward background current conductance gPflugers Archiv : European journal of physiology · 2020Article
- Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line.Journal of molecular and cellular cardiology · 2018Article
- Protein⁻Protein Interactions with Connexin 43: Regulation and Function.International journal of molecular sciences · 2018Review
- Connexins in Cardiovascular and Neurovascular Health and Disease: Pharmacological Implications.Pharmacological reviews · 2017Review
- Cardiac Cx43, Cx40 and Cx45 co-assembling: involvement of connexins epitopes in formation of hemichannels and Gap junction channels.BMC cell biology · 2017Review
- Pathogenesis and management of Brugada syndrome.Nature reviews. Cardiology · 2016Review
- Single-Cell Resolution of Temporal Gene Expression during Heart Development.Developmental cell · 2016Article
- The cardiac connexome: Non-canonical functions of connexin43 and their role in cardiac arrhythmias.Seminars in cell & developmental biology · 2016Review
- Nitrated fatty acids suppress angiotensin II-mediated fibrotic remodelling and atrial fibrillation.Cardiovascular research · 2016Article
- Scn1b deletion leads to increased tetrodotoxin-sensitive sodium current, altered intracellular calcium homeostasis and arrhythmias in murine hearts.The Journal of physiology · 2015Article
- Microdomain-specific localization of functional ion channels in cardiomyocytes: an emerging concept of local regulation and remodelling.Biophysical reviews · 2015Article
- Super-resolution imaging reveals that loss of the C-terminus of connexin43 limits microtubule plus-end capture and NaV1.5 localization at the intercalated disc.Cardiovascular research · 2014Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
aimsRemodelling and regional gradients in expression of connexins (Cx) are thought to contribute to atrial electrical dysfunction and atrial fibrillation. We assessed the effect of interaction between Cx43, Cx40, and Cx45 on atrial cell-to-cell coupling and inward Na current (I(Na)) in engineered pairs of atrial myocytes derived from wild-type mice (Cx43(+/+)) and mice with genetic ablation of Cx43 (Cx43(-/-)). METHODS AND
resultsCell pairs were engineered by microcontact printing from atrial Cx43(+/+) and Cx43(-/-) murine myocytes (1 day before birth, 3-5 days in culture). Dual and single voltage clamp were used to measure intercellular electrical conductance, g(j), and its dependence on transjunctional voltage, V(j), single gap junction channel conductances, and I(Na). 3D reconstructions of Cx43, Cx40, and Cx45 immunosignals in gap junctions were made from confocal slices. Full genetic Cx43 ablation produced a decrease in immunosignals of Cx40 to 62 ± 10% (mean ± SE; n= 17) and Cx45 to 66 ± 8% (n= 16). G(j) decreased from 80 ± 9 nS (Cx43(+/+), n= 17) to 24 ± 2 nS (Cx43(-/-), n= 35). Single channel analysis showed a shift in the main peak of the channel histogram from 49 ± 1.7 nS (Cx43(+/+)) to 67 ± 1.8 nS (Cx43(-/-)) with a second minor peak appearing at 27 ± 1.5 pS. The dependence of g(j) on V(j) decreased with Cx43 ablation. Importantly, peak I(Na) decreased from -350 ± 44 pA/pF (Cx43(+/+)) to -154 ± 28 pA/pF (Cx43(-/-)).
conclusionsThe dependence of Cx40, Cx45, and I(Na) on Cx43 expression indicates a complex interaction between connexins and I(Na) in the atrial intercalated discs that is likely to be of relevance for arrhythmogenesis.
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