ArticleJournal of bacteriology2012
Transcriptional and metabolomic consequences of LuxS inactivation reveal a metabolic rather than quorum-sensing role for LuxS in Lactobacillus reuteri 100-23.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Quorum Sensing via Autoinducer-2 Promotes Colonisation and Host Adaptation in B. bifidum PRL2010.Microbial biotechnology · 2025Article
- The human gut metacommunity as a conceptual aid in the development of precision medicine.Frontiers in microbiology · 2024Article
- Exploring Bacterial Attributes That Underpin Symbiont Life in the Monogastric Gut.Applied and environmental microbiology · 2022Review
- Nocturnal Acidification: A Coordinating Cue in theInternational journal of molecular sciences · 2022Review
- Amino Acids in Microbial Metabolism and Function.Advances in experimental medicine and biology · 2022Article
- Probiotics alter biofilm formation and the transcription ofJournal of oral microbiology · 2020Article
- Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure.Scientific reports · 2020Article
- Article
- Integrated Succinylome and Metabolome Profiling Reveals Crucial Role of S-Ribosylhomocysteine Lyase in Quorum Sensing and Metabolism ofMolecular & cellular proteomics : MCP · 2019Article
- The Progress of Multi-Omics Technologies: Determining Function in Lactic Acid Bacteria Using a Systems Level Approach.Frontiers in microbiology · 2019Review
- LuxS-dependent AI-2 production is not involved in global regulation of natural product biosynthesis inPeerJ · 2017Article
- Stress as a Normal Cue in the Symbiotic Environment.Trends in microbiology · 2016Review
- Lactobacillus reuteri 100-23 modulates urea hydrolysis in the murine stomach.Applied and environmental microbiology · 2014Article
- Diversity, ecology and intestinal function of bifidobacteria.Microbial cell factories · 2014Review
- Altered transcription of murine genes induced in the small bowel by administration of probiotic strain Lactobacillus rhamnosus HN001.Applied and environmental microbiology · 2014Article
- Article
- Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.PloS one · 2014Article
- A chemical biology approach to interrogate quorum-sensing regulated behaviors at the molecular and cellular level.Chemistry & biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autoinducer-2 (AI-2)-mediated quorum sensing has been extensively studied in relation to the regulation of microbial behavior. There are, however, two potential roles for the AI-2 synthase (LuxS). The first is in the production of AI-2 and the second is as an enzyme in the activated methyl cycle, where it catalyzes the conversion of S-ribosylhomocysteine to homocysteine. The by-product of the reaction catalyzed by LuxS is (S)-4,5-dihydroxy-2,3-pentanedione, which spontaneously forms the furanones known collectively as AI-2. The mammalian gut contains a complex collection of bacterial species so a method of interspecies communication might influence community structure and function. Lactobacillus reuteri 100-23 is an autochthonous inhabitant of the rodent forestomach, where it adheres to the nonsecretory epithelium, forming a biofilm. Microarray comparisons of gene expression profiles of the L. reuteri 100-23 wild type and a luxS mutant under different culture conditions revealed altered transcription of genes encoding proteins associated with cysteine biosynthesis/oxidative stress response, urease activity, and sortase-dependent proteins. Metabolomic analysis showed that the luxS mutation affected cellular levels of fermentation products, fatty acids and amino acids. Cell density-dependent changes (log phase versus stationary phase growth) in gene transcription were not detected, indicating that AI-2 was unlikely to be involved in gene regulation mediated by quorum sensing in L. reuteri 100-23.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.