ArticleApplied and environmental microbiology2012
Comprehensive set of integrative plasmid vectors for copper-inducible gene expression in Myxococcus xanthus.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 31 citations in OpenAlex.
- Secretins of type-two secretion systems are necessary for exopolymeric slime secretion in cyanobacteria and myxobacteria.Nature communications · 2025Article
- Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation.Nature communications · 2025Article
- Myxobacterial Genomics and Post-Genomics: A Review of Genome Biology, Genome Sequences and Related 'Omics Studies.Microorganisms · 2021Review
- MYXO-CTERM sorting tag directs proteins to the cell surface via the type II secretion system.Molecular microbiology · 2020Article
- Copper and Melanin Play a Role inFrontiers in microbiology · 2020Article
- Metal-responsive RNA polymerase extracytoplasmic function (ECF) sigma factors.Molecular microbiology · 2019Review
- Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus.Microbial cell factories · 2019Article
- Engineering Pseudochelin Production in Myxococcus xanthus.Applied and environmental microbiology · 2018Article
- Genome Editing in Model StrainBiomolecules · 2018Article
- Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus.Journal of visualized experiments : JoVE · 2018Article
- MotAB-like machinery drives the movement of MreB filaments during bacterial gliding motility.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.Microbial cell factories · 2018Article
- Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus.Nature communications · 2017Article
- Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus.Microbial cell factories · 2017Article
- Molecular toolbox for genetic manipulation of the stalked budding bacterium Hyphomonas neptunium.Applied and environmental microbiology · 2015Article
- Article
- Flagella stator homologs function as motors for myxobacterial gliding motility by moving in helical trajectories.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Tracking of chromosome and replisome dynamics in Myxococcus xanthus reveals a novel chromosome arrangement.PLoS genetics · 2013Article
- Two systems for conditional gene expression in Myxococcus xanthus inducible by isopropyl-β-D-thiogalactopyranoside or vanillate.Journal of bacteriology · 2012Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myxococcus xanthus is widely used as a model system for studying gliding motility, multicellular development, and cellular differentiation. Moreover, M. xanthus is a rich source of novel secondary metabolites. The analysis of these processes has been hampered by the limited set of tools for inducible gene expression. Here we report the construction of a set of plasmid vectors to allow copper-inducible gene expression in M. xanthus. Analysis of the effect of copper on strain DK1622 revealed that copper concentrations of up to 500 μM during growth and 60 μM during development do not affect physiological processes such as cell viability, motility, or aggregation into fruiting bodies. Of the copper-responsive promoters in M. xanthus reported so far, the multicopper oxidase cuoA promoter was used to construct expression vectors, because no basal expression is observed in the absence of copper and induction linearly depends on the copper concentration in the culture medium. Four different plasmid vectors have been constructed, with different marker selection genes and sites of integration in the M. xanthus chromosome. The vectors have been tested and gene expression quantified using the lacZ gene. Moreover, we demonstrate the functional complementation of the motility defect caused by lack of PilB by the copper-induced expression of the pilB gene. These versatile vectors are likely to deepen our understanding of the biology of M. xanthus and may also have biotechnological applications.
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