ArticleApplied and environmental microbiology2012
Construction and characterization of a gradually inducible expression vector for Halobacterium salinarum, based on the kdp promoter.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 17 citations in OpenAlex.
- Genomic insights into Serratia bockelmanii strain 201: unlocking its potential for plant growth promotion and salt stress tolerance.BMC microbiology · 2026Article
- Haloarchaea as Promising Chassis to Green Chemistry.Microorganisms · 2024Review
- Heterotrophic nitrification and related functional gene expression characteristics of Alcaligenes faecalis SDU20 with the potential use in swine wastewater treatment.Bioprocess and biosystems engineering · 2021Article
- Microbial Regulation of Deterioration and Preservation of Salted Kelp under Different Temperature and Salinity Conditions.Foods (Basel, Switzerland) · 2021Article
- Novel Enzymes From the Red Sea Brine Pools: Current State and Potential.Frontiers in microbiology · 2021Review
- A Synthetic Riboswitch to Regulate Haloarchaeal Gene Expression.Frontiers in microbiology · 2021Article
- In silico and experimental improvement of bacteriorhodopsin production in Halobacterium salinarum R1 by increasing DNA-binding affinity of Bat through Q661R/Q665R substitutions in HTH motif.Extremophiles : life under extreme conditions · 2019Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. For Halobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that the kdp promoter (Pkdp), which facilitates gene expression upon K(+) limitation, can be used to establish such a system for molecular applications. Pkdp features a rather high expression rate, with an approximately 50-fold increase that can be easily varied by K(+) concentrations in the growth medium. Besides the construction of an expression vector, our work describes the characterization of expression patterns and, thus, offers a gradually inducible expression system to the scientific community.
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