ArticleApplied and environmental microbiology2012
Supplementation of intracellular XylR leads to coutilization of hemicellulose sugars.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Pathway remodeling and adaptive evolution enable efficient co-utilization of glucose and xylose inmBio · 2026Article
- XylR Overexpression in Escherichia coli Alleviated Transcriptional Repression by Arabinose and Enhanced Xylitol Bioproduction from Xylose Mother Liquor.Applied biochemistry and biotechnology · 2024Article
- Validating a Xylose Regulator to Increase Polyhydroxybutyrate Production for Utilizing Mixed Sugars from Lignocellulosic Biomass UsingJournal of microbiology and biotechnology · 2024Article
- Development of an E. coli strain for cell-free ADC manufacturing.Biotechnology and bioengineering · 2022Article
- Engineering xylose metabolism for production of polyhydroxybutyrate in the non-model bacterium Burkholderia sacchari.Microbial cell factories · 2018Article
- Engineering E. coli for simultaneous glucose-xylose utilization during methyl ketone production.Microbial cell factories · 2018Article
- Article
- Metabolic Engineering Strategies for Co-Utilization of Carbon Sources in Microbes.Bioengineering (Basel, Switzerland) · 2016Review
- Reciprocal Regulation of l-Arabinose and d-Xylose Metabolism in Escherichia coli.Journal of bacteriology · 2016Article
- Next generation biofuel engineering in prokaryotes.Current opinion in chemical biology · 2013Review
- Structures of the Escherichia coli transcription activator and regulator of diauxie, XylR: an AraC DNA-binding family member with a LacI/GalR ligand-binding domain.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli has the potential to be a powerful biocatalyst for the conversion of lignocellulosic biomass into useful materials such as biofuels and polymers. One important challenge in using E. coli for the transformation of biomass sugars is diauxie, or sequential utilization of different types of sugars. We demonstrate that, by increasing the intracellular levels of the transcription factor XylR, the preferential consumption of arabinose before xylose can be eliminated. In addition, XylR augmentation must be finely tuned for robust coutilization of these two hemicellulosic sugars. Using a novel technique for scarless gene insertion, an additional copy of xylR was inserted into the araBAD operon. The resulting strain was superior at cometabolizing mixtures of arabinose and xylose and was able to produce at least 36% more ethanol than wild-type strains. This strain is a useful starting point for the development of an E. coli biocatalyst that can simultaneously convert all biomass sugars.
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