ArticleMolecular & cellular proteomics : MCP2012
Evaluation and properties of the budding yeast phosphoproteome.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Estimating the total number of phosphoproteins and phosphorylation sites in eukaryotic proteomes.GigaScience · 2017Pooled it
- A comprehensive proteome and the first phosphoproteome reveal extensive phosphorylation of carbohydrate metabolism in Cryptosporidium parvum sporozoites.Parasites & vectors · 2026Article
- From Petri Plates to Petri Nets, a revolution in yeast biology.FEMS yeast research · 2022Article
- In-depth and 3-dimensional exploration of the budding yeast phosphoproteome.EMBO reports · 2021Article
- Article
- Mutually exclusive locales for N-linked glycans and disorder in human glycoproteins.Scientific reports · 2020Article
- Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.Nucleic acids research · 2018Article
- Phosphorylation-mediated Regulatory Networks in Mycelia ofMolecular & cellular proteomics : MCP · 2017Article
- The Pivotal Role of Protein Phosphorylation in the Control of Yeast Central Metabolism.G3 (Bethesda, Md.) · 2017Article
- Article
- Untargeted metabolomics unravels functionalities of phosphorylation sites in Saccharomyces cerevisiae.BMC systems biology · 2016Article
- Activation and inhibition of Snf1 kinase activity by phosphorylation within the activation loop.Biochimica et biophysica acta · 2016Article
- DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.Genetics · 2016Article
- Prioritizing functional phosphorylation sites based on multiple feature integration.Scientific reports · 2016Article
- Site-directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia.European journal of histochemistry : EJH · 2016Article
- Intrinsic Disorder in Transmembrane Proteins: Roles in Signaling and Topology Prediction.PloS one · 2016Article
- Comparison of phosphorylation patterns across eukaryotes by discriminative N-gram analysis.BMC bioinformatics · 2015Article
- Analysis of the Candida albicans Phosphoproteome.Eukaryotic cell · 2015Article
- Association between intrinsic disorder and serine/threonine phosphorylation in Mycobacterium tuberculosis.PeerJ · 2015Article
- Proteomic analyses reveal that Sky1 modulates apoptosis and mitophagy in Saccharomyces cerevisiae cells exposed to cisplatin.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
We have assembled a reliable phosphoproteomic data set for budding yeast Saccharomyces cerevisiae and have investigated its properties. Twelve publicly available phosphoproteome data sets were triaged to obtain a subset of high-confidence phosphorylation sites (p-sites), free of "noisy" phosphorylations. Analysis of this combined data set suggests that the inventory of phosphoproteins in yeast is close to completion, but that these proteins may have many undiscovered p-sites. Proteins involved in budding and protein kinase activity have high numbers of p-sites and are highly over-represented in the vast majority of the yeast phosphoproteome data sets. The yeast phosphoproteome is characterized by a few proteins with many p-sites and many proteins with a few p-sites. We confirm a tendency for p-sites to cluster together and find evidence that kinases may phosphorylate off-target amino acids that are within one or two residues of their cognate target. This suggests that the precise position of the phosphorylated amino acid is not a stringent requirement for regulatory fidelity. Compared with nonphosphorylated proteins, phosphoproteins are more ancient, more abundant, have longer unstructured regions, have more genetic interactions, more protein interactions, and are under tighter post-translational regulation. It appears that phosphoproteins constitute the raw material for pathway rewiring and adaptation at various evolutionary rates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.