ArticleVirulence
Expression of Escherichia coli virulence usher protein attenuates wild-type Salmonella.
Article in Virulence. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Progress and perspectives in plant sterol and plant stanol research.Nutrition reviews · 2018Review
- Expression of periplasmic chaperones in Salmonella Typhimurium reduces its viability in vivo.Current microbiology · 2015Article
- Regulatory T-cell vaccination independent of auto-antigen.Experimental & molecular medicine · 2014Review
- Attenuating gene expression (AGE) for vaccine development.Virulence · 2013Review
- Adhesion protein ApfA of Actinobacillus pleuropneumoniae is required for pathogenesis and is a potential target for vaccine development.Clinical and vaccine immunology : CVI · 2013Article
- Role of overexpressed CFA/I fimbriae in bacterial swimming.Physical biology · 2012Article
- Flagella overexpression attenuates Salmonella pathogenesis.PloS one · 2012Article
- Article
- Coming of AGE: facile generation of attenuated vaccine strains through heterologous gene expression.VirulenceArticle
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11 authors.
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Abstract
Generation of a live attenuated vaccine for bacterial pathogens often requires prior knowledge of the pathogen's virulence factors. We hypothesized an alternative approach of heterologous gene expression would make a wild-type (wt) pathogen more susceptible to host cell killing, thus, resulting in immunization. As proof of concept, the heterologous expression of enterotoxigenic E. coli (ETEC) colonization factor antigen I (CFA/I) was tested to attenuate Salmonella. The overexpression of CFA/I resulted in significant attenuation of wt Salmonella. In-depth studies revealed the attenuation depended on the co-expression of chaperone (CfaA) and usher (CfaC) proteins. Remarkably, the CfaAC-attenuated Salmonella conferred protection against wt Salmonella challenge. Mechanistic study indicated CfaAC made Salmonella outer membranes permeable, causing Salmonella to be vulnerable to host destruction. Thus, enhancing bacterial permeability via CfaAC represents an alternative method to attenuate pathogens despite the presence of unknown virulence factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.