Evidence map›Paper›PMID 22286688›Full record

ArticleJournal of structural and functional genomics2012

Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.

Heungbok Kim, Cecelia Webster, Justin K M Roberts, Juthamas Kositsawat, Li-Wei Hung, Thomas C Terwilliger, Chang-Yub Kim

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural and functional genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Heungbok KimBioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Cecelia Webster
Justin K M Roberts
Juthamas Kositsawat
Li-Wei Hung
Thomas C Terwilliger
Chang-Yub Kim
Los Alamos National Laboratory · USLoma Linda University · USUniversity of California, Riverside · USUniversity of California System · US

Funding

Subproject 5U54GM074946 · NIGMS · UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB · PI TERWILLIGER, THOMAS C. · 2005 to 2009
$20.2M
NIGMS NIH HHS U54 GM 074946NIGMS NIH HHS U54 GM074946
6 · The paper itself

Abstract

Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.

Indexed as

Bacterial ProteinsChromatography, AffinityCrystallography, X-RayElectrophoresis, Polyacrylamide GelEscherichia coliLigandsMycobacterium tuberculosisNADP TranshydrogenasesNucleotidesProtein Interaction MappingRecombinant ProteinsTriazinesBacterial ProteinsCibacron Blue F 3GALigandsNADP TranshydrogenasesNucleotidesRecombinant ProteinsTriazines

Identifiers

PMID22286688
PMCPMC3375012
OpenAlexW2138193220

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.