ArticleAnalytical and bioanalytical chemistry2012
Dynamic monitoring of glucagon secretion from living cells on a microfluidic chip.
Article in Analytical and bioanalytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon.Electrophoresis · 2024Article
- Monitoring hormone and small molecule secretion dynamics from islets-on-chip.Analytical and bioanalytical chemistry · 2023Article
- Advances in microfluidic chips based on islet hormone-sensing techniques.World journal of diabetes · 2023Review
- Type 1Micromachines · 2023Review
- Visualizing hypoxic modulation of beta cell secretions via a sensor augmented oxygen gradient.Microsystems & nanoengineering · 2023Article
- Sex Differences in the Molecular Programs of Pancreatic Cells Contribute to the Differential Risks of Type 2 Diabetes.Endocrinology · 2022Article
- Islet-on-a-chip for the study of pancreatic β-cell function.In vitro models · 2022Review
- A microfluidic system for monitoring glucagon secretion from human pancreatic islets of Langerhans.Analytical methods : advancing methods and applications · 2021Article
- A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.Frontiers in bioengineering and biotechnology · 2021Article
- Pancreas-on-a-Chip Technology for Transplantation Applications.Current diabetes reports · 2020Review
- In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution.Advanced biosystems · 2020Article
- A microfluidic platform integrating pressure-driven and electroosmotic-driven flow with inline filters for affinity separations.Analytical methods : advancing methods and applications · 2019Article
- 3D-Models of Insulin-Producing β-Cells: from Primary Islet Cells to Stem Cell-Derived Islets.Stem cell reviews and reports · 2018Review
- A microfluidic in-line ELISA for measuring secreted protein under perfusion.Biomedical microdevices · 2017Article
- Time-Resolved Pharmacological Studies using Automated, On-line Monitoring of Five Parallel Suspension Cultures.Scientific reports · 2017Article
- Resealable, optically accessible, PDMS-free fluidic platform for ex vivo interrogation of pancreatic islets.Lab on a chip · 2017Article
- 3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling.Lab on a chip · 2017Article
- Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry.Analytical and bioanalytical chemistry · 2017Article
- Multiplexing Fluorescence Anisotropy Using Frequency Encoding.Analytical chemistry · 2016Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A rapid microfluidic based capillary electrophoresis immunoassay (CEIA) was developed for on-line monitoring of glucagon secretion from pancreatic islets of Langerhans. In the device, a cell chamber containing living islets was perfused with buffers containing either high or low glucose concentration. Perfusate was continuously sampled by electroosmosis through a separate channel on the chip. The perfusate was mixed on-line with fluorescein isothiocyanate-labeled glucagon (FITC-glucagon) and monoclonal anti-glucagon antibody. To minimize sample dilution, the on-chip mixing ratio of sampled perfusate to reagents was maximized by allowing reagents to only be added by diffusion. Every 6 s, the reaction mixture was injected onto a 1.5-cm separation channel where free FITC-glucagon and the FITC-glucagon-antibody complex were separated under an electric field of 700 V cm(-1). The immunoassay had a detection limit of 1 nM. Groups of islets were quantitatively monitored for changes in glucagon secretion as the glucose concentration was decreased from 15 to 1 mM in the perfusate revealing a pulse of glucagon secretion during a step change. The highly automated system should be enable studies of the regulation of glucagon and its potential role in diabetes and obesity. The method also further demonstrates the potential of rapid CEIA on microfluidic systems for monitoring cellular function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.