ArticleGene2012
Analysis of the 5' untranslated region (5'UTR) of the alcohol oxidase 1 (AOX1) gene in recombinant protein expression in Pichia pastoris.
Article in Gene, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Pichia pastoris Random Mutagenesis and Screening.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Novel transcriptional regulation of the GAP promoter in Pichia pastoris towards high expression of heterologous proteins.Microbial cell factories · 2024Article
- Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.Microbial cell factories · 2024Article
- Genetic tools for metabolic engineering ofEngineering microbiology · 2023Review
- Engineering of Promoters for Gene Expression in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Improved Production ofCurrent issues in molecular biology · 2021Article
- Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris.Microbial cell factories · 2018Article
- Article
- Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species.ACS synthetic biology · 2017Article
- An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast.Scientific reports · 2015Article
- Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris.Microbial cell factories · 2015Article
- Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.Applied microbiology and biotechnology · 2014Review
- Synthetic core promoters for Pichia pastoris.ACS synthetic biology · 2014Article
- The immediate upstream region of the 5'-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana.Nucleic acids research · 2014Article
- Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins.Biotechnology letters · 2013Article
- Combinatorial mutagenesis and selection to understand and improve yeast promoters.BioMed research international · 2013Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
Pichia pastoris is a methylotrophic yeast that has been genetically engineered to express over one thousand heterologous proteins valued for industrial, pharmaceutical and basic research purposes. In most cases, the 5' untranslated region (UTR) of the alcohol oxidase 1 (AOX1) gene is fused to the coding sequence of the recombinant gene for protein expression in this yeast. Because the effect of the AOX1 5'UTR on protein expression is not known, site-directed mutagenesis was performed in order to decrease or increase the length of this region. Both of these types of changes were shown to affect translational efficiency, not transcript stability. While increasing the length of the 5'UTR clearly decreased expression of a β-galactosidase reporter in a proportional manner, a deletion analysis demonstrated that the AOX1 5'UTR contains a complex mixture of both positive and negative cis-acting elements, suggesting that the construction of a synthetic 5'UTR optimized for a higher level of expression may be challenging.
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Registered trials
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