ArticleBiomaterials2012
The regulation of focal adhesion complex formation and salivary gland epithelial cell organization by nanofibrous PLGA scaffolds.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 95 citations in OpenAlex.
- Three-Dimensional Culture of Epithelial Cells on Electrospun Nanofibrous Scaffolds.International journal of molecular sciences · 2025Review
- Human iPSC-derived salivary gland organoids model diabetic salivary gland dysfunction.bioRxiv : the preprint server for biology · 2025Article
- Article
- Current basic and preclinical research for treatment of radiation therapy-induced hyposalivation.JADA foundational science · 2025Article
- Biomimetic Catechol-Incorporated Polyacrylonitrile Nanofiber Scaffolds for Tissue Engineering of Functional Salivary Glands.Biomaterials research · 2025Article
- Salivary Gland Bioengineering.Bioengineering (Basel, Switzerland) · 2023Review
- Salivary gland bioengineering - yesterday, today, tomorrow!Histology and histopathology · 2023Review
- Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.Biointerphases · 2023Article
- Hydrogels for Salivary Gland Tissue Engineering.Gels (Basel, Switzerland) · 2022Review
- Raman microspectroscopy fingerprinting of organoid differentiation state.Cellular & molecular biology letters · 2022Article
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
- Single-cell RNA sequencing reveals PDGFRα+ stromal cell subpopulations that promote proacinar cell differentiation in embryonic salivary gland organoids.Development (Cambridge, England) · 2022Article
- Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation.Bioengineering (Basel, Switzerland) · 2022Article
- Decellularized small intestine submucosa/polylactic-co-glycolic acid composite scaffold for potential application in hypopharyngeal and cervical esophageal tissue repair.Regenerative biomaterials · 2021Article
- Steering cell behavior through mechanobiology in 3D: A regenerative medicine perspective.Biomaterials · 2021Review
- Building a Functional Salivary Gland for Cell-Based Therapy: More than Secretory Epithelial Acini.Tissue engineering. Part A · 2020Review
- Mechanosensitive regulation of stanniocalcin-1 by zyxin and actin-myosin in human mesenchymal stromal cells.Stem cells (Dayton, Ohio) · 2020Article
- Smart Hydrogels in Tissue Engineering and Regenerative Medicine.Materials (Basel, Switzerland) · 2019Review
- The relationship between substrate topography and stem cell differentiation in the musculoskeletal system.Cellular and molecular life sciences : CMLS · 2019Review
- Extracellular matrix alignment dictates the organization of focal adhesions and directs uniaxial cell migration.APL bioengineering · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Nanofiber scaffolds have been useful for engineering tissues derived from mesenchymal cells, but few studies have investigated their applicability for epithelial cell-derived tissues. In this study, we generated nanofiber (250 nm) or microfiber (1200 nm) scaffolds via electrospinning from the polymer, poly-l-lactic-co-glycolic acid (PLGA). Cell-scaffold contacts were visualized using fluorescent immunocytochemistry and laser scanning confocal microscopy. Focal adhesion (FA) proteins, such as phosphorylated FAK (Tyr397), paxillin (Tyr118), talin and vinculin were localized to FA complexes in adult cells grown on planar surfaces but were reduced and diffusely localized in cells grown on nanofiber surfaces, similar to the pattern observed in adult mouse salivary gland tissues. Significant differences in epithelial cell morphology and cell clustering were also observed and quantified, using image segmentation and computational cell-graph analyses. No statistically significant differences in scaffold stiffness between planar PLGA film controls compared to nanofibers scaffolds were detected using nanoindentation with atomic force microscopy, indicating that scaffold topography rather than mechanical properties accounts for changes in cell attachments and cell structure. Finally, PLGA nanofiber scaffolds could support the spontaneous self-organization and branching of dissociated embryonic salivary gland cells. Nanofiber scaffolds may therefore have applicability in the future for engineering an artificial salivary gland.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.