ArticleJournal of agricultural and food chemistry2012
Homodimeric β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081: expression in Lactobacillus plantarum and biochemical characterization.
Article in Journal of agricultural and food chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 78 citations in OpenAlex.
- A GH42 β‑Galactosidase from the Human IsolateACS omega · 2025Article
- Characterization of a β-Galactosidase fromMolecules (Basel, Switzerland) · 2025Article
- Prebiotic Effects of α- and β-Galactooligosaccharides: The Structure-Function Relation.Molecules (Basel, Switzerland) · 2025Review
- Isolation and characterization of a β-galactosidase fromJDS communications · 2025Article
- Galacto-oligosaccharide production byJDS communications · 2024Article
- In Vitro Production of Galactooligosaccharides by a Novel β-Galactosidase ofInternational journal of molecular sciences · 2022Article
- Heterologous Protein Production in Lactobacillus (plantarum) Using pSIP Vectors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- β-galactosidase GALA fromBioengineered · 2021Article
- Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by β-Galactosidases from Lactic Acid Bacteria and Bifidobacteria.Journal of agricultural and food chemistry · 2020Article
- Safety Aspects of Genetically Modified Lactic Acid Bacteria.Microorganisms · 2020Review
- Immobilization of β-Galactosidases on theCatalysts (Basel, Switzerland) · 2019Article
- β-Galactosidase fromInternational journal of molecular sciences · 2019Article
- Technological Aspects of the Production of Fructo and Galacto-Oligosaccharides. Enzymatic Synthesis and Hydrolysis.Frontiers in nutrition · 2019Review
- Immobilization of β-Galactosidases from Lactobacillus on Chitin Using a Chitin-Binding Domain.Journal of agricultural and food chemistry · 2017Article
- From by-product to valuable components: Efficient enzymatic conversion of lactose in whey using β-galactosidase fromBiochemical engineering journal · 2016Article
- Engineering a thermostable Halothermothrix orenii β-glucosidase for improved galacto-oligosaccharide synthesis.Applied microbiology and biotechnology · 2016Article
- Transferase Activity of Lactobacillal and Bifidobacterial β-Galactosidases with Various Sugars as Galactosyl Acceptors.Journal of agricultural and food chemistry · 2016Article
- A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals.Biotechnology for biofuels · 2016Article
- Investigation of Film with β-Galactosidase Designed for Stabilization and Handling in Dry Configuration.Molecules (Basel, Switzerland) · 2015Article
- Fractionation of a galacto-oligosaccharides solution at low and high temperature using nanofiltration.Separation and purification technology · 2015Article
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Authors and funding
8 authors at 6 institutions in 5 countries.
Funding
Abstract
The lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, encoding a β-galactosidase of the glycoside hydrolase family GH2, was cloned into different inducible lactobacillal expression vectors for overexpression in the host strain Lactobacillus plantarum WCFS1. High expression levels were obtained in laboratory cultivations with yields of approximately 53000 U of β-galactosidase activity per liter of medium, which corresponds to ~170 mg of recombinant protein per liter and β-galactosidase levels amounting to 63% of the total intracellular protein of the host organism. The wild-type (nontagged) and histidine-tagged recombinant enzymes were purified to electrophoretic homogeneity and further characterized. β-Galactosidase from L. bulgaricus was used for lactose conversion and showed very high transgalactosylation activity. The maximum yield of galacto-oligosaccharides (GalOS) was approximately 50% when using an initial concentration of 600 mM lactose, indicating that the enzyme can be of interest for the production of GalOS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.