ArticleArteriosclerosis, thrombosis, and vascular biology2012
Flow cytometric identification and functional characterization of immature and mature circulating endothelial cells.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed.
- Multidimensional Proteomic Approach of Endothelial Progenitors Demonstrate Expression of KDR Restricted to CD19 Cells.Stem cell reviews and reports · 2021Trial
- Role of circulating endothelial cells in assessing the severity of systemic sclerosis and predicting its clinical worsening.Scientific reports · 2021Trial
- Heat therapy promotes the expression of angiogenic regulators in human skeletal muscle.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Trial
- Isolation of functional human endothelial cells from small volumes of umbilical cord blood.Annals of biomedical engineering · 2013Trial
- Reprogramming the Aging Type H Vascular Niche via Myeloid Angiogenic Cell-Derived Extracellular Vesicles: Mechanisms, Engineering Strategies, and Translational Perspectives.International journal of molecular sciences · 2026Review
- Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony-Forming Cells.Advanced healthcare materials · 2026Article
- Substrate-Dependent Variability in Viability and Angiogenic Marker Expression Among Three Endothelial Cell Subtypes: Insights for Artificial Tissue Vascularization.Macromolecular bioscience · 2025Article
- Human endothelial colony forming cells (ECFCs) require endothelial protein C receptor (EPCR) for cell cycle progression and angiogenic activity.Angiogenesis · 2025Article
- Article
- Augmenting the Angiogenic Profile and Functionality of Cord Blood Endothelial Colony-Forming Cells by Indirect Priming with Bone-Marrow-Derived Mesenchymal Stromal Cells.Biomedicines · 2023Article
- Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans.JCI insight · 2023Article
- Pathological Roles for Endothelial Colony-Forming Cells in Neonatal and Adult Lung Disease.American journal of respiratory cell and molecular biology · 2023Review
- Human Endothelial Colony-Forming Cells.Cold Spring Harbor perspectives in medicine · 2022Article
- Endothelialization and Inflammatory Reactions After Intracardiac Device Implantation.Advances in experimental medicine and biology · 2022Review
- Vascular Stem/Progenitor Cells in Vessel Injury and Repair.Frontiers in cardiovascular medicine · 2022Review
- Current concepts on endothelial stem cells definition, location, and markers.Stem cells translational medicine · 2021Review
- Article
- Endothelial Colony-Forming Cells Dysfunctions Are Associated with Arterial Hypertension in a Rat Model of Intrauterine Growth Restriction.International journal of molecular sciences · 2021Article
- Evaluation of circulating tumor cells in colorectal cancer using flow cytometry.The Journal of international medical research · 2021Article
- Proteomic Signature of Dysfunctional Circulating Endothelial Colony-Forming Cells of Young Adults.Journal of the American Heart Association · 2021Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
objectiveWe sought to identify and characterize 2 distinct populations of bona fide circulating endothelial cells, including the endothelial colony-forming cell (ECFC), by polychromatic flow cytometry (PFC), colony assays, immunomagnetic selection, and electron microscopy. METHODS AND
resultsMononuclear cells from human umbilical cord blood and peripheral blood were analyzed using our recently published PFC protocol. A population of cells containing both ECFCs and mature circulating endothelial cells was determined by varying expressions of CD34, CD31, and CD146 but not AC133 and CD45. After immunomagnetic separation, these cells failed to form hematopoietic colonies, yet clonogenic endothelial colonies with proliferative potential were obtained, thus verifying their identity as ECFCs. The frequency of ECFCs were increased in cord blood and were extremely rare in the peripheral blood of healthy adults. We also detected another mature endothelial cell population in the circulation that was apoptotic. Finally, when comparing this new protocol with a prior method, we determined that the present protocol identifies circulating endothelial cells, whereas the earlier protocol identified extracellular vesicles.
conclusionsTwo populations of circulating endothelial cells, including the functionally characterized ECFC, are now identifiable in human cord blood and peripheral blood by PFC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.