ArticleRNA (New York, N.Y.)2012
Deep-sequencing of endothelial cells exposed to hypoxia reveals the complexity of known and novel microRNAs.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 131 citations in OpenAlex.
- Cross-organ protection of MSC-derived extracellular vesicles in ischemia-reperfusion injury: angiogenic synergy in kidney, brain, and heart.Frontiers in cardiovascular medicine · 2025Review
- Research progress on the mechanism of angiogenesis in wound repair and regeneration.Frontiers in physiology · 2023Review
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- Overview of non-coding RNAs in breast cancers.Translational oncology · 2022Review
- Non-canonical features of microRNAs: paradigms emerging from cardiovascular disease.Nature reviews. Cardiology · 2022Review
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- A Non-Canonical Link between Non-Coding RNAs and Cardiovascular Diseases.Biomedicines · 2022Review
- Regulatory Non-coding RNAs in Atherosclerosis.Handbook of experimental pharmacology · 2022Review
- Issues of origin, morphology and clinical significance of tumor microvessels in gastric cancer.World journal of gastroenterology · 2021Review
- Endothelial Dysfunction in Pregnancy Complications.Biomedicines · 2021Review
- Impact of DICER1 and DROSHA on the Angiogenic Capacity of Human Endothelial Cells.International journal of molecular sciences · 2021Article
- Profiling of Primary and Mature miRNA Expression in Atherosclerosis-Associated Cell Types.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- MicroRNA regulation of cholesterol metabolism.Annals of the New York Academy of Sciences · 2021Review
- miR-130a activates the VEGFR2/STAT3/HIF1α axis to potentiate the vasoregenerative capacity of endothelial colony-forming cells in hypoxia.Molecular therapy. Nucleic acids · 2021Article
- Hypoxia re-programs 2'-O-Me modifications on ribosomal RNA.iScience · 2021Article
- MicroRNA-494 Regulates Endoplasmic Reticulum Stress in Endothelial Cells.Frontiers in cell and developmental biology · 2021Article
- Altered microRNA dynamics in acute coronary syndrome.Postepy w kardiologii interwencyjnej = Advances in interventional cardiology · 2020Article
- Metabolic labeling of RNA uncovers the contribution of transcription and decay rates on hypoxia-induced changes in RNA levels.RNA (New York, N.Y.) · 2020Article
- Factors Involved in miRNA Processing Change Its Expression Level during Imitation of Hypoxia in BeWo b30 Cells.Doklady. Biochemistry and biophysics · 2020Article
- Comprehensive transcriptomic profiling reveals SOX7 as an early regulator of angiogenesis in hypoxic human endothelial cells.The Journal of biological chemistry · 2020Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to understand the role of microRNAs (miRNAs) in vascular physiopathology, we took advantage of deep-sequencing techniques to accurately and comprehensively profile the entire miRNA population expressed by endothelial cells exposed to hypoxia. SOLiD sequencing of small RNAs derived from human umbilical vein endothelial cells (HUVECs) exposed to 1% O₂ or normoxia for 24 h yielded more than 22 million reads per library. A customized bioinformatic pipeline identified more than 400 annotated microRNA/microRNA* species with a broad abundance range: miR-21 and miR-126 totaled almost 40% of all miRNAs. A complex repertoire of isomiRs was found, displaying also 5' variations, potentially affecting target recognition. High-stringency bioinformatic analysis identified microRNA candidates, whose predicted pre-miRNAs folded into a stable hairpin. Validation of a subset by qPCR identified 18 high-confidence novel miRNAs as detectable in independent HUVEC cultures and associated to the RISC complex. The expression of two novel miRNAs was significantly down-modulated by hypoxia, while miR-210 was significantly induced. Gene ontology analysis of their predicted targets revealed a significant association to hypoxia-inducible factor signaling, cardiovascular diseases, and cancer. Overexpression of the novel miRNAs in hypoxic endothelial cells affected cell growth and confirmed the biological relevance of their down-modulation. In conclusion, deep-sequencing accurately profiled known, variant, and novel microRNAs expressed by endothelial cells in normoxia and hypoxia.
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Registered trials
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