ArticleInternational journal of legal medicine2012
Vaginal microbial flora analysis by next generation sequencing and microarrays; can microbes indicate vaginal origin in a forensic context?
Article in International journal of legal medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Advances in body fluid identification: MiRNA markers as powerful tool.International journal of legal medicine · 2024Pooled it
- Recent advances in the identification of vaginal fluid for forensic applications.International journal of legal medicine · 2026Review
- Characterization of vaginal microbiota diversity by 16S rRNA high-throughput sequencing.Frontiers in microbiology · 2026Article
- New Strategies for Preventing Perinatal Group B Streptococcus (GBS) Infections.Pathogens (Basel, Switzerland) · 2025Review
- Standardizing a microbiome pipeline for body fluid identification from complex crime scene stains.Applied and environmental microbiology · 2025Article
- Development and application of a multiplex PCR system for forensic salivary identification.International journal of legal medicine · 2023Article
- Microbiome-Based Stain Analyses in Crime Scenes.Applied and environmental microbiology · 2023Review
- Trends in forensic microbiology: From classical methods to deep learning.Frontiers in microbiology · 2023Review
- On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime.Genes · 2021Review
- Evaluation of CRISPR Diversity in the Human Skin Microbiome for Personal Identification.mSystems · 2021Article
- Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid.Diagnostics (Basel, Switzerland) · 2020Review
- Dietary α-Linolenic Acid-Rich Flaxseed Oil Exerts Beneficial Effects on Polycystic Ovary Syndrome Through Sex Steroid Hormones-Microbiota-Inflammation Axis in Rats.Frontiers in endocrinology · 2020Article
- An in-depth survey of the microbial landscape of the walls of a neonatal operating room.PloS one · 2020Article
- Specific microbes of saliva and vaginal fluid of Guangdong Han females based on 16S rDNA high-throughput sequencing.International journal of legal medicine · 2019Article
- Cohort Profile: African Collaborative Center for Microbiome and Genomics Research's (ACCME's) Human Papillomavirus (HPV) and Cervical Cancer Study.International journal of epidemiology · 2017Article
- Microbial population analysis improves the evidential value of faecal traces in forensic investigations.International journal of legal medicine · 2017Article
- Article
- Discrimination among individuals using terminal restriction fragment length polymorphism profiling of bacteria derived from forensic evidence.International journal of legal medicine · 2015Article
- Article
- Body fluid identification by integrated analysis of DNA methylation and body fluid-specific microbial DNA.International journal of legal medicine · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forensic analysis of biological traces generally encompasses the investigation of both the person who contributed to the trace and the body site(s) from which the trace originates. For instance, for sexual assault cases, it can be beneficial to distinguish vaginal samples from skin or saliva samples. In this study, we explored the use of microbial flora to indicate vaginal origin. First, we explored the vaginal microbiome for a large set of clinical vaginal samples (n = 240) by next generation sequencing (n = 338,184 sequence reads) and found 1,619 different sequences. Next, we selected 389 candidate probes targeting genera or species and designed a microarray, with which we analysed a diverse set of samples; 43 DNA extracts from vaginal samples and 25 DNA extracts from samples from other body sites, including sites in close proximity of or in contact with the vagina. Finally, we used the microarray results and next generation sequencing dataset to assess the potential for a future approach that uses microbial markers to indicate vaginal origin. Since no candidate genera/species were found to positively identify all vaginal DNA extracts on their own, while excluding all non-vaginal DNA extracts, we deduce that a reliable statement about the cellular origin of a biological trace should be based on the detection of multiple species within various genera. Microarray analysis of a sample will then render a microbial flora pattern that is probably best analysed in a probabilistic approach.
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