ArticleCurrent biology : CB2012
Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Yeast with elevated chromosome numbers are addicted to high levels of Mps1.bioRxiv : the preprint server for biology · 2025Article
- Phosphorylation of Mad1 at serine 18 by Mps1 is required for the full virulence of rice blast fungus, Magnaporthe oryzae.Molecular plant pathology · 2024Article
- Phosphorylation of Bub1 by Mph1 promotes Bub1 signaling at the kinetochore to ensure accurate chromosome segregation.The Journal of biological chemistry · 2024Article
- Leader of the SAC: molecular mechanisms of Mps1/TTK regulation in mitosis.Open biology · 2018Review
- TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability.British journal of pharmacology · 2017Article
- Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.Current biology : CB · 2017Article
- Dual recognition of chromatin and microtubules by INCENP is important for mitotic progression.The Journal of cell biology · 2017Article
- Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.Cell reports · 2017Article
- Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.PLoS genetics · 2016Article
- A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset.Biology open · 2015Article
- Signalling dynamics in the spindle checkpoint response.Nature reviews. Molecular cell biology · 2014Review
- Determinants of robustness in spindle assembly checkpoint signalling.Nature cell biology · 2013Article
- Review
- Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation.The EMBO journal · 2013Article
- Making an effective switch at the kinetochore by phosphorylation and dephosphorylation.Chromosoma · 2013Review
- A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.Cell cycle (Georgetown, Tex.) · 2013Article
- Microtubule attachment and spindle assembly checkpoint signalling at the kinetochore.Nature reviews. Molecular cell biology · 2013Review
- Kinetochore localized Mad2 and Cdc20 is itself insufficient for triggering the mitotic checkpoint when Mps1 is low in Drosophila melanogaster neuroblasts.Cell cycle (Georgetown, Tex.) · 2012Article
- MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.Nature cell biology · 2012Article
- Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1.Molecular biology of the cell · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Defects in chromosome segregation result in aneuploidy, which can lead to disease or cell death [1, 2]. The spindle checkpoint delays anaphase onset until all chromosomes are attached to spindle microtubules in a bipolar fashion [3, 4]. Mad2 is a key checkpoint component that undergoes conformational activation, catalyzed by a Mad1-Mad2 template enriched at unattached kinetochores [5]. Mad2 and Mad3 (BubR1) then bind and inhibit Cdc20 to form the mitotic checkpoint complex (MCC), which binds and inhibits the anaphase promoting complex (APC/C). Checkpoint kinases (Aurora, Bub1, and Mps1) are critical for checkpoint signaling, yet they have poorly defined roles and few substrates have been identified [6-8]. Here we demonstrate that a kinase-dead allele of the fission yeast MPS1 homolog (Mph1) is checkpoint defective and that levels of APC/C-associated Mad2 and Mad3 are dramatically reduced in this mutant. Thus, MCC binding to fission yeast APC/C is dependent on Mph1 kinase activity. We map and mutate several phosphorylation sites in Mad2, producing mutants that display reduced Cdc20-APC/C binding and an inability to maintain checkpoint arrest. We conclude that Mph1 kinase regulates the association of Mad2 with its binding partners and thereby mitotic arrest.
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